全輔助孵化優於局部輔助孵化以提高胚胎懷孕著床率

胚胎雷射輔助孵化，
全中空透明帶(ZP)輔助孵化似乎優於局部ZP輔助孵化
較明顯提高胚胎懷孕著床率

http://humupd.oxfordjournals.org/content/11/4/425.full

Possible applications of a non-contact 1.48 μm wavelength diode laser in assisted reproduction technologies

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Abstract

Recently, one laser system has been introduced in IVF fulfilling all safety requirements, while achieving a high standard of reproducibility in terms of ablation diameter. This 1.48 μm wavelength indium-gallium-arsenic-phosphorus (InGaAsP) semiconductor laser offers a variety of laser applications to the embryologist. On the one hand, zona pellucida of oocytes or embryos can be manipulated in order to facilitate ICSI or biopsy and assist hatching, and on the other, spermatozoa may be paralysed or immobilized prior to usage. To conclude, the 1.48 μm diode laser provides a promising tool for the microdissection of subcellular targets. The diode laser stands out due to the rapidity, the simplicity and the safety of the procedure which is supported by healthy offspring after laser application.

Figure 1(A).

Day 2 embryo after laser-assisted total hatching. Outer diameter of ablation is approximately 50 μm. (B) Day 2 embryo after laser-assisted partial hatching. Arrow indicates intact zona layer (8 μm). Outer diameter of ablation is approximately 45 μm. (C) Day 2 embryo after laser-assisted zona thinning. Area of thinning is approximately 80 μm.

Table I.

Technical deTechnical details of prospective studies on ltails of prospective studies on laser-assisted hatching

Authors	Day	Number of laser shots	Irradiation time (ms)	Size (μm) of opening	Hatching mode
Montag and van der Ven (1999)	2	2	18	15–35	Total
Baruffi et al. (2000)	2	1	12	16–18	Partial
Blake et al. (2001)	3	∼6	nd	80	Thinning
Mantoudis et al. (2001)	2	2	20	∼25	Total
Mantoudis et al. (2001)	2	∼7	20	>100	Thinning
Ebner et al. (2002a)	2, 3	3	12	35	Partial
Hsieh et al. (2002)	3	nd	5–10	5–10	Total
Petersen et al. (2002)	2, 3	1–2	10	15–20	Partial
Ali et al. (2003)	2, 3	nd	25	15–17	Total
Moser et al. (2003)	1	7–10	6	∼70	Thinning
Wong et al. (2003)	2, 3	3	2	∼23	Partial
Wong et al. (2003)	2, 3	2–4	2	∼21	Partial
Primi et al. (2004)	2, 3	1–4	12–30	20	Total

• Size of opening corresponds to the outer ablation; nd=no data available.