液態氮 vs 液態氮氣(vapor)用於精蟲保存效果類似

液態氮vs 液態氮氣(vapor)用於精蟲保存效果類似



Fertil Steril. 2015 Aug 3. pii: S0015-0282(15)01651-9. doi: 10.1016/j.fertnstert.2015.07.1140. [Epub ahead of print]

Liquid nitrogen vapor is comparable to liquid nitrogen for storage of cryopreserved human sperm: evidence from the characteristics of post-thaw human sperm.

Hu J1, Zhao S1, Xu C1, Zhang L1, Lu S1, Cui L1, Ma J2, Chen ZJ3.

Author information

Abstract

OBJECTIVE:

To compare the differences in the characteristics of post-thaw human sperm after storage in either liquid nitrogen (LN2; -196°C) or LN2 vapor (-167°C).

DESIGN:

Experimental study.

SETTING:

University hospital.

PATIENT(S):

Thirty healthy volunteers who agreed to donate their normal semen samples for infertility or research were included in the study.

INTERVENTION(S):

Semen samples (n = 30) were divided into eight aliquots and frozen. Four aliquots of each human semen sample were stored in LN2 (-196°C), and the other four aliquots were stored in LN2 vapor (-167°C). After 1, 3, 6, or 12 months, samples were thawed and analyzed.

MAIN OUTCOME MEASURE(S):

The motility was evaluated by the manual counting method. The viability was estimated by eosin staining. The morphology was analyzed by Diff-Quik staining. The sperm DNA integrity was determined with acridine orange fluorescent staining, and acrosin activity was assayed by the modified Kennedy method.

RESULT(S):

The characteristics of post-thaw human sperm, including motility, viability, morphology, DNA integrity, and acrosin activity, showed no significant difference between LN2 and LN2 vapor storage for the different time periods.

CONCLUSION(S):

LN2 vapor was comparable to LN2 in post-thaw sperm characteristics, suggesting that LN2 vapor may be substituted for LN2 for the long-term storage of human sperm.