濾泡太小(<15mm, vs >18mm)所取得卵子受孕率下降(47 % vs. 67 %)，異常受孕率上升(53% vs 27%)

J Assist Reprod Genet. 2014 Jan;31(1):73-7. doi: 10.1007/s10815-013-0124-9. Epub 2013 Nov 6.

Correlation between follicular diameters and flushing versus no flushing on oocyte maturity, fertilization rate and embryo quality.

Mehri S1, Levi Setti PE, Greco K, Sakkas D, Martinez G, Patrizio P.

Author information

1

Yale University Medical School-Yale Fertility Center, New Haven, CT, USA.

Abstract

OBJECTIVE:

To determine (a) the correlation between follicular sizes, oocyte maturity, normal fertilization rate, cleavage and embryo quality; and (b) to establish whether oocytes recovered with or without follicular flushing have different developmental competence.

DESIGN:

Prospective observational study.

SETTING:

Academic medical center.

PATIENTS:

Forty nine cycles (37 ICSI and 12 IVF).

INTERVENTIONS:

Measurement of 360 follicular diameters on the day of egg retrieval and classification into three groups Group A (mean diameter 12-14.5 mm.), group B (mean diameter 15-18 mm.) and group C (diameter >18.5 mm.).

MAIN OUTCOME MEASURE:

Correlation between follicular size at the time of retrieval and oocyte maturity, fertilization and cleavage rate in 226 oocytes (163 ICSI and 63 IVF). Developmental competence of oocytes retrieved with flushing versus non flushing.

RESULTS:

Almost all (99 %) of the oocytes recovered from follicles of group C were in metaphase II as opposed to 80 % in group A and 81 % in group B (p < 0.01). Overall there was a progressive and significant increase in fertilization rates from group A follicles to group C (47 % vs. 67 %, p 0.05). Overall 53 % of oocytes retrieved from group A follicles showed either no fertilization or abnormal fertilization versus 27 % in group C (p 0.05). The oocyte recovery rate with follicular flushing improved from group A to group B and to group C follicles (65 % vs. 49 % vs.37 % respectively p < 0.01). There were no differences in rates of immature oocyte, fertilization, abnormal or not fertilization and cleavage.

CONCLUSIONS:

The results of this study shows that: a) Follicles larger than 18 mm at retrieval have consistently mature oocytes with a higher rate of fertilization; b) Small size follicles are still capable of containing mature oocytes, but their rate of abnormal or no fertilization is high; c) Oocytes recovered with flushing are still able to produce embryos with full developmental competence.