精蟲與20%濾泡液共同培養可提升精蟲活動力&下降精蟲DNA異常率

J Assist Reprod Genet. 2019 Dec 5. doi: 10.1007/s10815-019-01643-2. [Epub ahead of print]

Co-incubation of spermatozoa with human follicular fluid reduces sperm DNA fragmentation by mitigating DNase activity in the seminal plasma.

Dorado-Silva M1, Bartolomé-Nebreda J2, Sánchez-Martín P1, Johnston S3, Gosálvez J2.

Author information

1

Ginemed, Clinic for Assisted Reproduction, Sevilla, Spain.

2

Unit of Genetics, Department of Biology, Universidad Autónoma de Madrid, Madrid, Spain.

3

School of Agriculture and Food Sciences, University of Queensland, Gatton, Australia. s.johnston1@uq.edu.au.

Abstract

PURPOSE:

To examine the effect of co-incubating spermatozoa with human follicular fluid (HFF) on the rate of sperm DNA fragmentation.

METHODS:

This prospective study used semen (n = 23) and HFF from oocyte donors (n = 23). Liquified semen was divided into four aliquots: (1) neat semen (NEAT), (2) seminal plasma removed and replaced with sperm media (HTF) containing 0% (FF0), (3) 20% (FF20), or (4) 50% (FF50) HFF. Sperm motility and DNA fragmentation (SDF) were assessed following 24 h of incubation at 37 °C. Pro-oxidant capacity of HFF and seminal plasma and the effect of HFF on seminal plasma DNase activity was assessed in a sub-sample of 10 ejaculates.

RESULTS:

Sperm motility was higher after 3 h of incubation in media that contained HFF compared to the NEAT sample or when sperm was diluted in media without HFF. r-SDF (increase of SDF per time unit) values after 24 h of incubation for NEAT, FF0, FF20 and FF50 were 0.91, 0.69, 0.25 and 0.36, respectively. While pro-oxidant capacity of seminal plasma samples showed large variation (mean: 94.6 colour units; SD 65.4), it was lower and more homogeneous in FF samples (mean: 29.9 colour units; SD: 6.3). Addition of HFF to seminal plasma appeared to inhibit DNase activity.

CONCLUSION:

While differences exist in the pro-oxidant capacity of seminal plasma of patients, sperm DNA integrity was preserved with addition of HFF to sperm media, irrespective of the level of pro-oxidant capacity. DNase activity in the original seminal plasma was abolished after HFF co-incubation.