2024年12月23日

囊胚切片和冷凍之間間隔時間過長會影響胚胎著床率

間隔時間超過 1 小時會顯著影響胚胎存活和臨床結果

. 2021 Aug;29(4):276-281.

doi: 10.1017/S0967199420000866. Epub 2021 Feb 18.

Prolonged interval time between blastocyst biopsy and vitrification compromised the outcomes in preimplantation genetic testing

These cycles were divided into three groups according to the interval times: <1 h group, 1-2 h group, and ≥2 h group.
In PGT-A cycles, the clinical pregnancy and ongoing pregnancy rates in the <1 h group were 67.44% and 53.49%, respectively, higher than that in the 1-2 h group (52.94%, 47.06%, P > 0.05) and the ≥2 h group (52.63%, 36.84%, P > 0.05).
In conclusion, vitrification of blastocysts beyond 1 h after biopsy significantly influences embryo survival and clinical outcomes and is therefore not recommended.

2024年12月21日

子宮移植現況

2014 年首次實現子宮移植產下嬰兒
子宮移植不涉及輸卵管移植，日後必須採行試管嬰兒治療
絕對子宮因素不孕症影響 1:500 育齡婦女
子宮移植術後併發症為9/54（17%），大多與泌尿系統相關, 如腎積水、輸尿管瘺和低滲透壓膀胱
髂內動脈與髂外側動脈端側吻合
子宮卵巢靜脈與髂外靜脈端側吻合。
最常見的併發症是早期移植失敗，23% 病例在移植後頭幾個月內發生移植失敗，移植失敗的主要原因是移植血管血栓或灌注不足，導致子宮壞死
另一個併發症是縫合陰道狹窄，通常在移植後幾個月發生。72%出現陰道狹窄，其中一半接受非手術擴張治療，其餘接受手術治療

2024年12月20日

鑑定精子缺陷睪丸切片中的差異表達基因

https://onlinelibrary.wiley.com/doi/full/10.1002/rmb2.12616

透過 RNA 定序 (RNA-Seq) 量化轉錄異常精子的睪丸組織中差異表達的轉錄本，從而揭示過濾差異表達基因（DEG）透過 qRT-PCR 驗證了選定的 10 個 DEG，並在睪丸活檢中確定了兩種蛋白質的定位。找出調節精子形態和功能的新基因。

分析揭示 6 個基因（SPATA31E1、TEKT3、SLC9C1、PDE4A、CFAP47 和 TNC）與人類精子發育高度相關

睪丸切片兩種蛋白 ORAI1 和 SPATA31E1 的免疫組織化學定位證實在發育中的人類生殖細胞中表達，其中 SPATA31E1 在晚期精母細胞和精子細胞表現明顯

此研究方法可找出了影響人類生殖細胞的基因及相關蛋白

Identification of differentially expressed genes in human testis biopsies with defective spermatogenesis

Methods

Human testis biopsies were collected from men with well-characterized phenotypes of normal spermatogenesis, spermatid arrest, and Sertoli cell-only phenotype, and transcriptional differences were quantified by RNA-sequencing (RNA-Seq). Differentially expressed genes (DEGs) were filtered based on predominant expression in spermatids and gene functional annotations relevant to sperm morphology and motility. Selected 10 DEGs were validated by qRT-PCR and the localization of two proteins was determined in testis biopsies.

Results

The analysis revealed 6 genes (SPATA31E1, TEKT3, SLC9C1, PDE4A, CFAP47, and TNC) that are excellent candidates for novel genes enriched in developing human sperm. The immunohistochemical localization of two proteins, ORAI1 and SPATA31E1, in testis biopsies, verified that both are expressed in developing human germ cells, with SPATA31E1 enriched in late spermatocytes and spermatids.