Laser可提高囊胚冷凍之存活率

玻璃化冷凍前，施行囊胚雷射輔助孵化可提高囊胚冷凍之存活率

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3114962/?report=reader

In vitro and in vivo viability of human blastocysts collapsed by laser pulse or osmotic shock prior to vitrification

Hiroshi Iwayama, Shinichi Hochi, and Masanori Yamashita

Additional article information

Abstract

Purpose

This study was designed to investigate whether artificial shrinkage, induced by a laser pulse or hyperosmotic sucrose solutions, improves in vitro survival and/or implantation of vitrified-warmed human expanded blastocysts.

Methods

Before Cryotop vitrification, the blastocoelic cavity was collapsed either by a laser pulse or sucrose solutions. Non-treated blastocysts were used as control. Post-warm blastocyst survival and implantation after transfer were examined. Implantation rate outcome was retrospectively analyzed by morphological grading and developmental kinetics of post-warm blastocysts.

Results

Survival rates in the three groups were high. Implantation rates in the laser-pulse group (59.7%) were comparable with those in the sucrose group (49.3%), and were significantly higher than those in the control group (34.2%). The proportion of blastocysts showing fast development tended to be higher when the blastocysts underwent artificial shrinkage treatment before vitrification. There was no clear correlation between morphology of post-warm blastocysts and implantation rate.

Conclusion

Artificial shrinkage treatment before vitrification is associated with an increased probability of fast-developing embryos, resulting in higher implantation rates.

Keywords: Artificial shrinkage, Clinical pregnancy, Laser pulse, Sucrose, Vitrification