3PN胚胎可存活於4度達24h
http://www.ncbi.nlm.nih.gov/pubmed/23820799
Short-term storage of tripronucleated human embryos.
PURPOSE:
To determine the survival and subsequent in vitro development of human cleavage stage embryos and hatched blastocysts following varying periods of short-term storage at 4 °C, using tripronucleated human embryos (TPN) as a model.
METHODS:
TPN cleavage embryos and hatched blastocysts short-term stored at 4 °C for 0 h (control), 24 h and 48 h. The main outcome measures were: survival rates (SR) and in vitro developmental ability (blastocyst rate and blastocyst-re-expansion rate) in each of the groups after storage.
RESULTS:
Cleavage-stage TPN survived at comparable rates to controls, regardless of storage time (average: 97.3 %). The in vitro development of cleavage-stage TPN stored for 24 h was comparable to that of controls (average 64.7 %), but was significantly impaired when storage lasted 48-h (20.8 %). After artificial shrinkage, SR was comparable in 24-h-stored and non-stored hatched blastocysts (85.7 %; p > 0.05), but was significantly impaired in the 48-h-stored group (20.0 %). Following 24-h storage, the re-expansion rate of hatched blastocysts was similar to that of controls (average: 57.1 %; p > 0.05), but was higher than that of the 48-h-stored group (15.0 %; p < 0.05).
CONCLUSIONS:
TPN human cleavage embryos and blastocysts can be successfully stored short-term for up to 24 h at 4 °C without using cryoprotectants without any significant negative impact on survival or subsequent in vitro development.
Table 1
Survival and subsequent in vitro developmental ability to progress to the blastocyst stage of cleavage –stage tripronucleated embryos after 4 °C storage for different time periods (0 h, 24 h and 48 h)
| 0 h | 24 h | 48 h |
|---|
| Initial number of embryos stored | 32 | 19 | 24 |
| Survival rate (n, %) | 32 (100) | 19 (100) | 22 (91.7) |
| Blastocyst rate (n, %) | 20 (62.5)a | 13 (68.4)a | 5 (20.8)b |
a,bDifferent superscripts within a row indicate
p
<
0.05
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