卵丘細胞之粒線體DNA狀況(DNA copy number)與卵細胞之品質呈正相關
The median mtDNA content of CCs for good- and poor-quality embryos was 140 and 57, respectively (p < 0.0001). 

J Assist Reprod Genet. 2016 Mar;33(3):367-71. doi: 10.1007/s10815-015-0621-0. Epub 2016 Jan 9.

Mitochondrial DNA copy number in cumulus cells is a strong predictor of obtaining good-quality embryos after IVF.

Ogino M1, Tsubamoto H1, Sakata K1, Oohama N2, Hayakawa H2, Kojima T1, Shigeta M2, Shibahara H3.

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Abstract

PURPOSE:

The aim of this study was to establish a simple tool to predict good-quality embryos in in vitro fertilization (IVF) by using cumulus cells (CCs) or peripheral blood cells (PBCs).

METHODS:

Mitochondrial DNA was extracted from CCs and PBCs in patients undergoing IVF. Using real-time polymerase chain reaction, mtDNA copy number in a single cell was calculated. Embryo quality was assessed when it was transferred or frozen.

RESULTS:

CCs were obtained from 60 oocyte cumulus-cell complexes (OCCCs) in 30 women, and PBCs were collected from 18 women. For the 30 women in the study, the median age was 37 years old (range, 24-43), and the mean body mass index was 21.4 (standard error, 2.0). mtDNA content of CCs and PBCs was highly correlated (Pearson's r = 0.900, p < 0.0001). The median mtDNA content of CCs for good- and poor-quality embryos was 140 and 57, respectively (p < 0.0001). The median mtDNA content of PBCs for good- and poor-quality embryos was 36 and 13, respectively (p = 0.604). The logistic regression model indicated that mtDNA content in CCs was the only parameter that predicted good-quality embryos (p = 0.020). The receiver operating characteristic curve for obtaining good-quality embryos by mtDNA copy number in CCs had an area under the curve of 0.823, and using a threshold of 86, positive and negative predictive values were 84.4 and 82.1 %, respectively.

CONCLUSIONS:

The determination of mtDNA content in CCs can be used to predict good-quality embryos.