2026年6月18日

 卵泡<14mm 卵子成熟率只有40-50%

 卵泡<14mm 取出的比率只有60-80%

https://www.rbmojournal.com/article/S1472-6483(25)00253-6/fulltext

Follicular diameterNo. of cyclesMedian oocyte retrieval rates (%)95% CI
≥11 mm42960.054.3–65.7
≥12 mm42966.762.5–70.8
≥13 mm42972.768.0–77.4
≥14 mm42982.676.6–88.5
≥15 mm429100.092.1–107.9
≥16 mm429103.3101.3–105.3
≥17 mm426133.3123.3–143.4
≥18 mm406175.0159.6–190.4
≥19 mm374250.0225.3–274.7
≥20 mm310300.0254.9–345.1
≥21 mm229333.3268.8–397.9
≥22 mm167400.0332.0–468.0
≥23 mm101400.0304.4–495.6
≥24 mm63400.0304.1–495.9
Table 2
Median oocyte retrieval rates
Median oocyte retrieval rates (95% CI) were measured by dividing the number of oocytes retrieved by the number of follicles measuring equal to or greater than each of the follicular diameters.
Bold type indicates statistical significance.




Follicular diameterNo. of cyclesMedian mature oocyte retrieval rates (%)95% CI
≥11 mm21842.939.4–46.3
≥12 mm21846.743.2–50.2
≥13 mm21850.046.1–53.9
≥14 mm21858.854.2–63.5
≥15 mm21866.762.1–71.2
≥16 mm21883.378.3–88.4
≥17 mm216100.090.9–109.1
≥18 mm207133.3112.5–154.2
≥19 mm188200.0176.4–223.6
≥20 mm158233.3200.3–266.4
≥21 mm117300.0228.1–371.9
≥22 mm80325.0236.5–413.5
≥23 mm47300.0235.3–364.7
≥24 mm27300.0173.7–426.3
Table 3
Median mature oocyte retrieval rates
Median mature oocyte retrieval rates (95% CI) were measured by dividing the number of mature oocytes retrieved by the number of follicles measuring equal to or greater than each of the follicular diameters.

胚胎培養O2濃度

 5% O2濃度  vs  逐步下降 oxygen(8%–2%)濃度

-----5% O2濃度可得較高囊胚率&品質




Effect of constant (5%) versus gradient (8%–2%) oxygen concentration on sibling human blastocyst development


Research question

Does a gradient of oxygen concentration, decreasing from 8% to 2% during prolonged embryo culture, yield a higher percentage of morphologically optimal blastocysts compared with a static 5% oxygen atmosphere?

Design

This prospective sibling-split study included 658 cumulus-oocyte complexes from 44 intracytoplasmic sperm injection (ICSI) cycles (January 2022 to January 2023), alternately assigned to a control group (static 5% oxygen) or an intervention group (stepwise oxygen reduction: 8% on days 0-3, 5% on day 3, and 2% until days 5 or 6).

Results

Early embryo morphology was similar between groups. By day 5, following exposure to 2% oxygen from day 3, the control group produced a significantly higher proportion of clinically used blastocysts (47.3%, 122/258 vs. 36.9%, 97/263; P = 0.0161). Rates of morphologically optimal day-5 blastocysts were comparable (14.7%, 38/258 vs. 10.3%, 27/263; P = 0.2373). Time-lapse analysis showed delayed initiation of blastulation (P = 0.0024), blastocyst formation (P < 0.001), and expansion (P < 0.001) in the intervention group. Because of this delay, embryologists were more likely to select blastocysts for fresh transfer from the control group in a blinded procedure (P = 0.0088). Morphometric analysis showed significantly larger blastocyst cross-sectional area in the control group (28,198 ± 4139 vs. 25,994 ± 4657 µm2; P = 0.0014), consistent with more advanced expansion under constant 5% oxygen.

Conclusions

Gradually lowering oxygen from 8% to 2% during the second half of embryo culture resulted in delayed blastulation and fewer clinically used blastocysts when compared with culture under static 5% oxygen.