Day 4 桑葚體胚胎之碎片比例(5%, 5%-20%, or >20%)與胚胎囊胚率( 57.4%, 50%, and 35.6%)有關

Fertil Steril. 2011 Dec;96(6):1473-1478.e2. doi: 10.1016/j.fertnstert.2011.09.015. Epub 2011 Oct 6.

Prediction of human blastocyst development from morulas with delayed and/or incomplete compaction.

Ivec M1, Kovacic B, Vlaisavljevic V.

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Abstract

OBJECTIVE:

To determine the influence of delayed compaction and fragmentation on the developmental capacity of morulas.

DESIGN:

Prospective study.

SETTING:

University IVF center.

PATIENT(S):

Intracytoplasmic sperm injection (ICSI) cycles with compact embryos on day 4 or day 5.

INTERVENTION(S):

The embryos were divided into day 4 (n = 329) and day 5 (n = 256) morulas and graded I, II, or III, according to the percentage of fragmentation (<5%, 5%-20%, or >20%). The embryos were measured using Cronus3 software.

MAIN OUTCOME MEASUREMENT(S):

Blastocyst development rate, blastocoel expansion rate, and optimal blastocyst rate. In an optimal blastocyst: surface area, trophectoderm cell number, inner cell mass (ICM) surface area, ICM volume and ICM shape.

RESULT(S):

Day 4 morulas in classes I-III developed into optimal blastocysts in 57.4%, 50%, and 35.6% of the total, respectively, and day 5 morulas in classes I-III in 43.3%, 29.1%, and 13.6% of the total, respectively. A negative association was identified between the amount of morula fragmentation, the blastocyst ICM size, and the number of trophectoderm cells. A delay of 1 day in compaction was associated with a reduced ICM volume.

CONCLUSION(S):

The measurement of compaction timing and cytoplasmic loss in morulas assists in predicting their ability to develop into optimal blastocysts.