Day3胚胎切片會延後胚胎形成桑葚體及囊胚之時間進而下降胚胎著床率

J Assist Reprod Genet. 2016 Oct 1. [Epub ahead of print]

Blastomere biopsy for PGD delays embryo compaction and blastulation: a time-lapse microscopic analysis.

Bar-El L1, Kalma Y1, Malcov M1, Schwartz T1, Raviv S1, Cohen T1, Amir H1, Cohen Y1, Reches A1, Amit A1, Ben-Yosef D2,3.

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Abstract

PURPOSE:

The purpose of the study was to explore the effect of blastomere biopsy for preimplantation genetic diagnosis (PGD) on the embryos' dynamics, further cleavage, development, and implantation.

METHODS:

The study group included 366 embryos from all PGD treatments (September 2012 to June 2014) cultured in the EmbryoScope™ time-lapse monitoring system. The control group included all intracytoplasmic sperm injection (ICSI) embryos cultured in EmbryoScope™ until day 5 during the same time period (385 embryos). Time points of key embryonic events were analyzed with an EmbryoViewer™.

RESULTS:

Most (88 %) of the embryos were biopsied at ≥8 cells. These results summarize the further dynamic development of the largest cohort of biopsied embryos and demonstrate that blastomere biopsy of cleavage-stage embryos significantly delayed compaction and blastulation compared to the control non-biopsied embryos. This delay in preimplanation developmental events also affected postimplantation development as observed when the dynamics of non-implanted embryos (known implantation data (KID) negative) were compared to those of implanted embryos (KID positive).

CONCLUSION:

Analysis of morphokinetic parameters enabled us to explore how blastomere biopsy interferes with the dynamic sequence of developmental events. Our results show that biopsy delays the compaction and the blastulation of the embryos, leading to a decrease in implantation.