Day 1單原核1PN之胚胎染色體正常比率僅17%
形成囊胚之比例<5%

J Assist Reprod Genet. 2017 May 11. doi: 10.1007/s10815-017-0937-z. [Epub ahead of print]

Could monopronucleated ICSI zygotes be considered for transfer? Analysis through time-lapse monitoring and PGS.

Mateo S1, Vidal F2, Parriego M3, Rodríguez I4, Montalvo V3, Veiga A3,5, Boada M3.

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Abstract

PURPOSE:

The purpose of this study was to investigate the chromosomal constitution and the developmental potential of intracytoplasmic sperm injection (ICSI) deriving embryos displaying a single pronucleus at the zygote stage.

METHODS:

Eighty-eight embryos from single pronucleus (1PN) two polar bodies (2PB) ICSI zygotes from 64 preimplantational genetic screening (PGS) cycles (October 2012-December 2014), were retrospectively analyzed. Zygotes were cultured in a time-lapse incubator. Embryo biopsy was performed on day 3 and genetic analysis approached by array comparative genomic hybridization.

RESULTS:

Chromosomal analysis revealed that 17% (15/88) of embryos derived from 1PN 2PB zygotes were diagnosed as euploid. After blastomere biopsy at day 3, the blastocyst rate at day 5 was 3.4% (3/88). Only 2.3% (2/88) euploid blastocysts were obtained. In two couples and after counseling and patient agreement, the transfer of a euploid blastocyst from a 1PN 2PB ICSI zygote was performed resulting in the birth of a healthy child.

CONCLUSIONS:

These results open the possibility to consider embryos coming from 1PN 2PB ICSI zygotes for transfer when no other embryos from 2PN 2PB ICSI zygotes are available and if a PGS diagnosis of euploidy is obtained. Confirmation of biparental inheritance is strongly recommended.