40 iu hyaluronidase使用於cumulus removal

40 iu/ml hyaluronidase使用於cumulus removal

recombinanet hyaluronidase vs. bovine hyaluronidase (Type VIII, Sigma Chemical Company, St Louis, MO, USA) 
效果類似

http://humrep.oxfordjournals.org/content/23/8/1815.long

Table I.

Baseline outcome measures.

	Cumulase® (n = 778 COCs)	Bovine hyaluronidase (n = 781 COCs)
Enzyme incubation timea	61.9 ± 21.0	62.8 ± 29.2
Mechanical denudationb	17.2 ± 7.6	16.8 ± 7.6
Availability for ICSIc	80.0 ± 21.4	78.9 ± 20.2

• COCs, cumulus–oocyte complexes.
• All values represent mean ± standard deviation (SD).
• ^aseconds.
• ^b× in/out pipetting.
• ^cMean percentage per cycle of metaphase-II oocytes expressed per number of COCs retrieved.

Table II.

Primary outcome measures.

	Cumulase® (n = 621 injected M-IIs)a	Bovine hyaluronidase (n = 611 injected M-IIs)a	Absolute difference in percentages between arms
ICSI intact oocytesb	89.6% (86.0–93.2)c	92.9% (90.5–95.3)c	−3.3% (−7.4 to 10.7)c
Fertilized oocytes (2-PN)b	73.9% (69.5–78.3)c	77.1% (73.3–80.9)c	−3.2% (−8.3 to 1.8)c

• ^aM-II, mature metaphase-II oocyte.
• ^bMean percentages per cycle, expressed per injected M-II oocyte.
• ^cValues between brackets indicate 95% confidence interval.

Table III.

Embryo development Day 3 and Day 5.

	Cumulase®	Bovine hyaluronidase	Absolute difference in percentages between arms
Day 3 developmenta ≥8-cell, ≤10% fragments	19.5 (14.8–24.1)b	22.4 (17.4–27.4)b	−2.9% (−8.2 to 2.4)b
Day 5 developmentaExcellent or good Q blastocyst	15.8 (10.1–21.5)b	13.1 (7.0–19.3)b	2.7% (−4.8 to 10.1)b

• ^aMean percentages per cycle, expressed per oocyte fertilized.
• ^bValues between brackets indicate 95% confidence interval.