IVF後6h未受孕卵施行rescue ICSI

IVF後6h 檢查PB以確定是否受孕
未受孕卵施行rescue ICSI, 懷孕率12%

http://www.ncbi.nlm.nih.gov/pubmed/16580372/

Fertil Steril. 2006 Apr;85(4):901-6.

Effect of reduced oocyte aging on the outcome of rescue intracytoplasmic sperm injection.

Nagy ZP1, Rienzi LF, Ubaldi FM, Greco E, Massey JB, Kort HI.

Author information

Abstract

OBJECTIVE:

To evaluate the results of a novel protocol that allows to rescue IVF unfertilized oocytes by intracytoplasmic sperm injection (ICSI).

DESIGN:

Prospective clinical trial.

SETTING:

Private reproductive medical center.

PATIENT(S):

Thirty patients undergoing IVF.

INTERVENTION(S):

Controlled ovarian stimulation (COS), conventional IVF, rescue ICSI, embryo culture, and embryo transfer.

MAIN OUTCOME MEASURE(S):

Identification of unfertilized IVF oocytes 6 hours after insemination and fertilization, and developmental rates of those oocytes after rescue microinjection, as well implantation and pregnancy rates (PR).

RESULT(S):

All oocytes (392) from 30 patients were inseminated with standard IVF 3 hours after ovum pick-up. Polar body (PB) status was checked at decumulation and rechecked 3 hours later. Eighty-two oocytes were fertilized after IVF alone and 184 nonactivated oocytes (failed fertilization) were rescue microinjected and 166 of them fertilized (20 patients). Cleavage stage on day 2 was significantly more advanced and embryo grade was higher after standard IVF fertilization than after rescue ICSI. Eight of the 30 embryos transferred were implanted in the IVF-only patients (27%) and 8 of 68 embryos in the rescue ICSI patients (12%).

CONCLUSION(S):

Rescue ICSI of unfertilized IVF oocytes 6 hours after insemination (9 hours after egg retrieval) can provide normal fertilization, embryo development, and pregnancy; however, corresponding outcome parameters tend to be impaired in comparison to the standard IVF fertilization results.