縮減囊胚腔體積可能可提高冷凍解凍懷孕率

縮減囊胚期胚胎之囊胚腔體積，可能可提高冷凍解凍懷孕率

http://www.rbej.com/content/7/1/99

縮減囊胚腔體積之方法

Different pre-vitrification interventions for blastocysts. A. Assisted hatching: An opening is created in the zona using laser pulse B. Needle blastocoele puncture: A needle is passed through the zona and blastocoele and retracted allowing the blastocelic fluid to freely leak. C. Laser blastocoele puncture: laser pulse creates an opening in the zona and a small defect in the trophectoderm causing the blastocoele to leak. D. Blastocoele aspiration: An injection needle is introduced into the blastocoele and blastocoelic volume is sucked out. E. Micropipetting: Passing the blastocysts through a narrow pipette would crack the zona and compress the blastocoele to leak through the cracked zona.

Kader et al. Reproductive Biology and Endocrinology 2009 7:99   doi:10.1186/1477-7827-7-99
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