蜂蜜& Vit E 有助於精蟲生長

動物實驗顯示，蜂蜜& Vit E 有助於精蟲生長

http://www.jhrsonline.org/article.asp?issn=0974-1208;year=2013;volume=6;issue=1;spage=54;epage=58;aulast=Hemadi

The effects of honey and vitamin E administration on apoptosis in testes of rat exposed to noise stress

Masoud Hemadi¹, Ghasem Saki², Asghar Rajabzadeh², Ali Khodadadi³, Alireza Sarkaki^2
1 Fertility, Infertility and Perinatology Research Center, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
² Physiology Research Center, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
³ Department of Immunology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Date of Submission	21-Jul-2012
Date of Decision	14-Sep-2012
Date of Acceptance	03-Dec-2012
Date of Web Publication	21-May-2013

Correspondence Address:
Ghasem Saki
Physiology Research Center, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, P.O. Box 61355 189, Ahvaz
Iran

DOI: 10.4103/0974-1208.112383
PMID: 23869153

Abstract

Aims: A variety of stress factors are known to inhibit male reproductive functions. So this study was conducted in order to investigate the effects of honey and vitamin E on the germinative and somatic cells of testes of rats exposed to noise stress. Materials and Methods:Mature male wistar rats (n0 = 24) were randomly grouped as follows: Group 1 (honey + noise stress), 2 (vitamin E + noise stress), 3 (noise stress,) and 4 as the control group. In groups 1, 2, and 3, rats were exposed to noise stress. In groups 1 and 2, rats also were given honey and vitamin E, respectively, orally for 50 days. After that, the germinative and somatic cells of testes parenchyma were isolated by digesting the whole testes by a standard method. Next, viability, apoptosis, and necrosis of the cells were evaluated by TUNEL kit and flow cytometry.Results: The rates of apoptosis and necrosis of the testicular cells were increased (P = 0.003 and P = 0.001, respectively), but viability of these cells decreased in testes of rats exposed to noise stress (P = 0.003). However, administration of honey and vitamin E were significantly helpful in keeping the cells of testis parenchyma alive, which suffers from noise pollution (P < 0.05 and P < 0.05, respectively).Conclusions: Noise stress has negative influences on the cells of testicular tissue by increasing apoptotic and necrotic cells. However, the associated enhancement in healthy cells suggests that honey and vitamin E have positive influences on the testis parenchyma.