hCG影響早期胎盤之發育

hCG影響早期懷孕(~8week)胎盤之發育


http://humrep.oxfordjournals.org/content/30/5/1029.abstract

Dynamic changes in hyperglycosylated human chorionic gonadotrophin throughout the first trimester of pregnancy and its role in early placentation

STUDY QUESTION What is the in situ localization and function of hyperglycosylated hCG (hCG-H) in first trimester pregnancy tissues?

SUMMARY ANSWER HCG-H localizes to the syncytiotrophoblast, cytotrophoblast and invasive extravillous trophoblast within the maternal decidua and promotes invasion during the first trimester of pregnancy.

WHAT IS KNOWN ALREADY Serum levels of hCG-H decline dramatically throughout the first trimester of pregnancy. As hCG-H is produced by choriocarcinoma cells, it is proposed to regulate trophoblast invasion.

STUDY DESIGN, SIZE, DURATION Tissues were collected from elective first trimester pregnancy terminations. Placental villous and decidua basalis were collected from Week 6 to Week 12 of gestation (n = 49).

PARTICIPANTS/MATERIALS, SETTING, METHODS Tissues were collected from elective first trimester surgical pregnancy terminations to determine localization, abundance and function of hCG-H. Placental villous outgrowth studies determined the impact of neutralizing endogenous hCG-H on trophoblast function. Real-time proliferation, migration and invasion assays using JEG-3 choriocarcinoma cells further elucidated the role of hCG-H in trophoblast function.

MAIN RESULTS AND THE ROLE OF CHANCE HCG-H localized to syncytiotrophoblast layer of the placental villous from gestational weeks 6–9; thereafter hCG-H localized as a discrete layer between syncytio- and cyto-trophoblast layers. Immunoreactive hCG-H was also observed within the cytotrophoblast layer in Week 7–8 of gestation. HCG-H abundance decreased within placental villous from Weeks 6–12 of gestation (n = 3 placentas per gestational weeks 6–12). HCG-H also localized to anchoring villi within maternal decidua, extravillous trophoblasts invading into the maternal decidua and endovascular trophoblasts remodeling maternal blood vessels. Treatment of primary first trimester villous explants with hCG-H neutralizing antibody reduced trophoblast outgrowth (n = 3 placentas, P < 0.05). Treatment of a trophoblast cell line with neutralizing antibody reduced trophoblast invasion (n = 4, P < 0.05) but did not affect migration or proliferation.