超細超薄毛細管(內徑200 μm, 厚20 μm.)用於玻璃化冷凍癌細胞(five human epithelial cancer cell lines (PC3: prostate cancer, T24: bladder cancer, SkBr-3: breast cancer, H1650: non-small-cell lung cancer, and HepG2: liver cancer) )
===>存活率90%

冷凍保存管管壁越薄, 管徑越細保存效果越佳

使用0.75PROH 3min-->離心5min--->1.5 M PROH and 0.5 M trehalose  3min 取代7.5%->15%DMSO+EG+0.5M sucrose玻璃冷凍

http://www.ncbi.nlm.nih.gov/pubmed/25914896

Figure 1

Evaluation of a fused silica microcapillary microcapillary as a tool for vitrification. (a) Map showing the heat transfer and cyoprotectant requirements for vitrification. The line indicated the relationship between the varying dimensions of microcapillary ...