卵丘細胞較少(Fig a)之卵子異常率較高應優先考慮施行ICSI

異常現象包括: ZP外觀異常薄, 外圍粗燥(Fig c), perivitelline space過小&PB無法分辨(Fig c), 細胞質無彈性(fig e)----應施行ICSI

GV>M2過程中perivitelline space 會擴大

perivitelline space過大過小卵子較易異常



Representative images of the abnormal and normal oocytes and embryos under light inverted microscopy. a The abnormal oocyte–corona-cumulus complex with less granular cumulus around the abnormal oocyte, and (b) the normal one with much granular cumulus surrounded. c The denuded oocyte with narrow perivitelline space (PVS), heterogeous zona pellucida (ZP) with less smooth outer edge, and hard to distinguished polar body compared with (d) the normal oocyte. e No resistance during ICSI being performed and no crease left after ICSI for abnormal oocyte compared with (f) the normal oocyte. g, h The morphology of two pronuclear (2PN) zygotes from abnormal and normal oocytes looked similar without consideration of PVS. i, k The cleaved embryos at 4–8 cell stage without clear blastomere interval for abnormal oocytes and (j, l) normal 4–8-cell embryos Original magnification: 200×


http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3947078/