傳統觀念建議禁慾3-5天  本篇發現禁慾1h 反而提升囊胚染色體正常率

J Assist Reprod Genet. 2019 Aug;36(8):1591-1597. doi: 10.1007/s10815-019-01533-7. Epub 2019 Jul 19.

How 1 h of abstinence improves sperm quality and increases embryo euploidy rate after PGT-A: a study on 106 sibling biopsied blastocysts.

Scarselli F1, Cursio E2, Muzzì S2, Casciani V2, Ruberti A2, Gatti S2, Greco P2, Varricchio MT2, Minasi MG2, Greco E2.

Author information

1

Centre for Reproductive Medicine, European Hospital, Via Portuense 700, 00149, Rome, Italy. filomenascarselli@virgilio.it.

2

Centre for Reproductive Medicine, European Hospital, Via Portuense 700, 00149, Rome, Italy.

Abstract

PURPOSE:

The aim of our study was to evaluate the influence of different ejaculatory abstinence time frames (several days versus 1 h) on semen parameters, blastocysts ploidy rate, and clinical results in assisted reproduction cycles on sibling oocytes.

METHODS:

This is a prospective study including 22 preimplantation genetic testing for aneuploidy (PGT-A) cycles performed between November 2015 and December 2018. Male partners with oligoastenoteratozoospermia produced two semen samples on the day of oocyte retrieval: the first one after several days of abstinence and the second, 1 h after the first one. Oocytes from each patient were divided into two groups: those in group 1 were injected with spermatozoa from the first ejaculate (N = 121) and oocytes in group 2 with spermatozoa from the second one (N = 144). Outcomes of aniline blue test, fertilization, blastocyst formation, ploidy rates, and clinical results were compared between the two groups.

RESULTS:

Semen volume resulted lower in the second sperm retrieval. Sperm concentration, motility, and morphology were similar in the two groups. A total of 106 blasotcysts were biospied. Higher blastocyst euploidy rates resulted in group 2 (43.6%) than in group 1 (27.5%). A higher percentage of mature chromatine was observed in group 2.

CONCLUSION:

Using spermatozoa from samples with a shorter abstinence could be a simple method to select higher quality spermatozoa, reducing aneuploidy rate in blastocysts. Prospective randomized controlled trials should be performed to confirm the potential advantage of using semen samples with short abstinence period to improve the outcome of assisted reproduction cycles.