精蟲冷凍 0.5-5 vs 6-10 vs 11-15 years
活產率:  81.63%, 79.11% and 73.91%,
流產率:  12.26%, 11.38% and 17.39%
精蟲冷凍超過5年可能對於精蟲品質也輕微不良影響

Fertil Steril. 2019 Jul 29. pii: S0015-0282(19)30505-9. doi: 10.1016/j.fertnstert.2019.06.008. [Epub ahead of print]

Long-term cryostorage of semen in a human sperm bank does not affect clinical outcomes.

Huang C1, Lei L2, Wu HL1, Gan RX1, Yuan XB2, Fan LQ3, Zhu WB4.

Author information

1

Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, Hunan, People's Republic of China.

2

Institute of Reproductive and Stem Cell Engineering, Basic Medicine College, Central South University, Hunan, People's Republic of China.

3

Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, Hunan, People's Republic of China; Institute of Reproductive and Stem Cell Engineering, Basic Medicine College, Central South University, Hunan, People's Republic of China.

4

Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, Hunan, People's Republic of China; Institute of Reproductive and Stem Cell Engineering, Basic Medicine College, Central South University, Hunan, People's Republic of China. Electronic address: zhuwenbing0971@sina.com.

Abstract

OBJECTIVE:

To evaluate the affect of the duration of donor sperm storage on pregnancy success among women undergoing assisted reproduction.

DESIGN:

Retrospective cross-sectional study.

SETTING:

Sperm bank.

PATIENT(S):

A total of 119,558 specimens retrieved using a clinical information database of young adult men who were qualified sperm donors at the Hunan Province Human Sperm Bank of China from 2001 to 2016.

INTERVENTION(S):

Analysis of semen samples and clinical outcomes after semen use.

MAIN OUTCOME MEASURE(S):

Clinical information included semen parameters before and after freezing, clinical pregnancy, abortion and live birth rates after semen use.

RESULT(S):

The sperm's frozen-thaw survival rate decreased from 85.72% to 73.98% after 15 years of cryopreservation (P<.01). The clinical pregnancy rate of women undergoing artificial insemination with donor sperm was 23.09%, 22.36% and 22.32%, the clinical abortion rate was 10.06%, 10.02% and 12.00% and the live birth rate was 82.17%, 80.21% and 80.00% in the groups with 0.5-5, 6-10 and 11-15 storage years, respectively. The clinical pregnancy rate of women undergoing in vitro fertilization was 64.29%, 64.94% and 53.48%, the clinical abortion rate was 12.26%, 11.38% and 17.39% and the live birth rate was 81.63%, 79.11% and 73.91%, in the groups with 0.5-5, 6-10 and 11-15 years, respectively.

CONCLUSION(S):

The long-term cryostorage of semen in a human sperm bank does not affect clinical outcomes. However, cryopreservation longer than 5 years negatively influenced the quality of frozen-thawed donor sperm samples.