paraffin oil vs mineral oil 應用於IVF胚胎培養, 胚胎品質& 懷孕率並無明顯差異

Syst Biol Reprod Med. 2019 Feb;65(1):81-86. doi: 10.1080/19396368.2018.1492645. Epub 2018 Jul 9.

Comparison between paraffin and mineral oil covering on early human embryo culture: a prospective randomized study.

Labied S1, Jouan C1, Wenders F1, Ravet S1, Gaspard O1, Thonon F1, Gridelet V1, Henry L1,2, Perrier d'Hauterive S1,2, Nisolle M1,2.

Author information

1

a Center for Assisted Medical Procreation University of Liège, CHR Citadelle , Liège , Belgium.

2

b Department of Obstetrics and Gynecology, CHR Citadelle , University of Liège , Liège , Belgium.

Abstract

The oil overlay in microdrop culture systems prevents medium evaporation, helps to maintain appropriate pH and osmotic conditions and protects from microbial contamination. In the present study, we prospectively compared covering by Ovoil™, a paraffin oil, and LiteOil®, a mineral oil, on the in vitro development of human embryos and their suitability for transfer/freezing at day 3 and live birth rate. One hundred and one patients undergoing in vitro fertilization (IVF) treatment by  intracytoplasmic sperm injection (ICSI) were enrolled in our study. After ICSI, 1237 oocytes were 1:1 randomly allocated into 2 groups according to the type of overlaying oil: Ovoil™ (616 oocytes) or LiteOil® (621 oocytes). Fertilization rate was assessed around 18 hours post-insemination (hpi) and embryos were checked for early cleavage at 25 hpi. Embryo morphology was recorded on days 2 and 3. A total of 437 (Ovoil™) and 438 day 3 embryos (LiteOil®) were analyzed. There were no differences between the two groups in terms of fertilization rate and occurrence of early cleavage. The proportion of top quality embryos (41.7% vs. 41.2%) and the final utilization rates (92.2% vs. 92.0%) were similar in Ovoil and LiteOil groups, respectively, at day 3. Live birth rate per transfer was essentially the same with Ovoil™ overlay (26.9%) when compared to LiteOil® (26.2%). Live birth rate in patients who simultaneously received  embryos from both overlay types was 17.2%. Despite the different characteristics of these two oils regarding hydrocarbon saturation, packing and temperature storage, Ovoil™ and LiteOil® can be used in parallel in the same IVF protocol. Abbreviations: ART: assisted reproductive technologies; hpi: hours post-insemination; hSA: human serum albumin; HTF: human tubal fluid; ICSI: intracytoplasmic sperm injection; IVF: in vitro fertilization; MII: metaphase II; MEA: mouse embryo assay; RT: room temperature.