IVF或ICSI前，將收集的卵子在特製培養基中培養3-6H，可提高卵子的成熟度和早期胚胎發育品質。

Review

 

. 2025 May 2;24(1):e12652.

 doi: 10.1002/rmb2.12652. eCollection 2025 Jan-Dec.

The quality of human eggs and its pre-IVF incubation

Background: Multi-factors influence the success rate of infertility treatments, and one of the important points is to obtain good quality eggs. Methods: Based on the literatures and unpublished data, the factors affecting egg quality were summarized.

Main findings results: Egg quality is an important determinant in successful infertility treatment. In addition to maternal age, controlled ovarian hyperstimulation (COH) protocols also play a key role in affecting the quality of the egg. After egg retrieval, the insemination occurs 3-6 h after collection, with a pre-IVF incubation time by in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) (39-42 h post-HCG injection). The pre-IVF incubation refers to the short period time of 3 to 6 h after oocyte retrieval and before the insemination by IVF or ICSI. The pre-IVF incubation of collected eggs in the designed culture medium improves egg quality in terms of maturation and early embryonic development.

Conclusions: Pre-IVF incubation of the collected eggs contributes to the improvement of the quality of eggs; therefore, it may increase subsequent pregnancy and implantation rates following embryo transfer.