80%胚胎在授精後8小時可觀察到原核
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3241845/
About 90% of fertilized oocytes released the second polar body at 6 h of insemination and 80% presented two pronuclei at 8 h of insemination [14–17]. We therefore chose a 6 h period of co-incubation.
J Assist Reprod Genet. 2013 Feb;30(2):251-7. doi: 10.1007/s10815-012-9922-8. Epub 2012 Dec 28.
Conventional IVF versus ICSI in sibling oocytes from couples with endometriosis and normozoospermic semen.
Komsky-Elbaz A, Raziel A, Friedler S, Strassburger D, Kasterstein E, Komarovsky D, Ron-El R, Ben-Ami I.
Source
IVF and Infertility unit, Assaf Harofeh Medical Center, Sackler School of Medicine, Tel Aviv University, Zerifin, Israel.
Abstract
PURPOSE:
This study compares the fertilization rate and embryonic development of oocytes randomly inseminated by conventional IVF or ICSI in patients with endometriosis and normozoospermic semen during IVF cycles.
METHODS:
Sibling oocytes were randomized to be inseminated either by ICSI or IVF. Rates of fertilization, cleavage, blastulation and embryonic morphology were assessed.
RESULTS:
A total of 786 sibling cumulus-oocyte complexes (COC) were randomized between insemination by conventional IVF (387 COC) or ICSI (399 COC). A significantly higher fertilization rate was found in the ICSI group (ICSI versus IVF, 73.3±23 % versus 54.7±31.9 % respectively; P=0.003), yielding a higher mean number of day 2 embryos (5.2±3.4 versus 3.6±2.9 respectively; P=0.002). Triploid fertilization rate (3PN/COC) was significantly higher in the IVF group compared to the ICSI group (3.9±8.7 % versus 0.9±3.1 % respectively; P=0.02). The morphology score and rate of development of day 2 and 3 embryos were not different between the two groups. Comparison of embryo transfer cycles in which either IVF or ICSI only embryos were transferred did not reveal any statistically significant differences in pregnancy or implantation rates.
CONCLUSION:
ICSI appears to be a better treatment option than conventional IVF in endometriosis-associated infertility, since it offers the advantages of higher fertilization rate and mean number of embryos and lower rate of total fertilization failure and triploid fertilization.
Comparison between early rescue ICSI cycles and late rescue ICSI cycles
| Early rescue ICSI | Late rescue ICSI | P-value | Power | |
|---|---|---|---|---|
| Patients | 80 | 3 | − | |
| Female age | 31.31 ±4.17 | 35.33±3.21 | NS | 0.56 |
| No.of oocytes retrieved | 9.66±4.93 | 9.33±5.19 | NS | 0.03 |
| No.of MII oocytes | 7.88±4.51 | 6.67±6.35 | NS | 0.05 |
| Sperm concentration 106 /ml | 59.54±23.52 | 55±36.35 | NS | 0.04 |
| Progressive sperm (%) | 42.01±9.52 | 39.67±6.66 | NS | 0.08 |
| IVF fertilization rate (%) | 8.12 | 0 | − | |
| ICSI fertilization rate (%) | 76.5 | 87.67 | NS | 0.05 |
| Mean No. of embryo transferred | 2.09±0.62 | 1.5±0.71 | NS | 0.29 |
| No.of ET cycles (n) | 70 | 2 | − | |
| Cycles of cancel ET (n) | 10 | 1 | − | |
| Cycle cancel reason | ||||
| Abnormal fertilization | 3 | 1 | − | |
| Unfertilized | 1 | 0 | − | |
| Froze due to uterine cavity fluid | 6 | 0 | − | |
| Clinical pregnancy rate (%) | 51.43% (36/70) | 0 | − | |
沒有留言:
張貼留言