HIV病患AIH採用多次離心法

HIV男性採用多次離心法(45–90% colloidal silica density gradient)+swim-up 清洗精蟲
分離HIV-free sperm from the infected NSC
降低精蟲感染HIV機率
統計顯示感染HIV機率低

http://humrep.oxfordjournals.org/content/25/8/1869.full

A decade of sperm washing: clinical correlates of successful insemination outcome

1. James D.M. Nicopoullos*, 
2. Paula Almeida, 
3. Maria Vourliotis, 
4. Rebecca Gouldingand 
5. Carole Gilling-Smith

+Author Affiliations

1. Chelsea and Westminster Assisted Conception Unit, 369 Fulham Rd, London SW10 9NH, UK

1. *Correspondence address. E-mail: jnicopoullos@aol.com

• Received January 19, 2010.
• Revision received April 24, 2010.
• Accepted April 30, 2010.

 

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Abstract

BACKGROUND Since 1999, we have treated HIV-positive men with sperm washing as part of a risk-reduction programme.

METHODS Retrospective analysis of the sperm-washing database from the treatment of 245 couples with 439 cycles of intrauterine insemination assessed the effects of patient factors (age, maternal FSH, rank of attempt), markers of HIV-disease [time since diagnosis, CD4 count, viral load (VL), use of highly active antiretroviral therapy (HAART)], cycle factors (natural versus stimulated, number of follicles, fresh versus frozen sperm) and sperm parameters on clinical (CPR) and ongoing pregnancy rate (OPR).

RESULTS Overall 111–245 (45.4%) couples achieved a clinical pregnancy (CPR: 13.5% and OPR: 9.6% per insemination) with no seroconversions. The mean duration since HIV diagnosis was 5.8 years, 73% of men were on antiretroviral therapy, there was an undetectable VL in 64% and the median CD4 was 409 cells/mm³. A significantly decreased OPR and a non-significantly increased miscarriage rate (MR) was observed after the female age of 40. Similarly, there was a significant increased OPR and decreased MR for women with a mean cycle maternal FSH of <6.4 IU/l. There was no effect of VL, CD4 count, use of HAART or time since diagnosis on the outcome. Nor was there a difference in the OPR according to paternal age, rank of attempt, cycle regime or number of follicles. Semen volume, sperm concentration, total count and progressive motility and post-wash concentration, progressive motility and total motile count inseminated were significantly higher in successful cycles. The use of frozen sperm had a significant negative impact on outcome.

CONCLUSIONS This study of the potential safe and successful reproductive options available to HIV-positive men demonstrates that maternal age and semen quality, rather than HIV factors, remain the most important determinants of cycle success.