不含glucose & phosphate 培養液有利胚胎生長分裂

不含glucose & phosphate 分裂期胚胎培養液(P1 Mediu, Irvine Scientific)
----仍達相同受孕率
----有利胚胎生長分裂

Composition of P1 Medium (Irvine Scientific)

NaHCO3  25 mM

Na-pyruvate 0.33 mM

Na-lactate 21.4 mM

Human serum 20%SSS is 1% albumin (hSA) equivalent to 1% hSA Amino acids

Taurine   0.05 mM

Na-citrate   0.15 mg/L

Phenol red   0.005 g/L

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3455441/pdf/10815_2004_Article_494970.pdf

Prospective Randomized Comparison of Two Embryo Culture Systems: P1 Medium by Irvine Scientific and the Cook IVF Medium

Purpose : To compare the efficacy of two commercially available in vitro fertilization (IVF) and embryo culture media systems: the glucose-free P1 Medium supplemented with 20% synthetic serum substitute (SSS) (Irvine Scientific), and the Cook IVF Medium (Cook, Australia). Methods: A prospective randomized study. Medical center-based IVF Unit affiliated to the Faculty of Medicine of Tel Aviv University. IVF patients were randomly assigned to either P1 Medium supplemented with 20% SSS (182 patients, 196 cycles) or Cook Medium (167 patients, 179 cycles). Results: Fertilization rates were similar with both media (52.3 ± 26.1 and 53.8 ± 27.6, respectively). Likewise, no difference was found in morphological characteristics and grading of cultured embryos. However, a significantly higher proportion of the embryos incubated in the P1 Medium reached the four-cell stage on day 2 or the 6-cell stage on day 3 postfertilization, compared to those incubated in Cook Medium (54.3% vs. 41.9%, p < 0.0001). Clinical pregnancy and delivery rates were improved when oocytes and embryos were cultured in P1 Medium. Finally, Implantation rate was significantly higher in the P1 Medium Group (9.9% vs. 6%, respectively). Conclusions: Our results suggest that the P1 Medium may be associated with a higher embryo cleavage rate and improved implantation rates compared to the Cook IVF Medium