胚胎植入過程若植入速度太快, 壓力太高, 均可能會下降胚胎品質及植床率
Pressure induced nucleus DNA fragmentation.
Abstract
PURPOSE:
The present study was designed to investigate the impact of pressure on nuclear DNA integrity in viable cells of mouse blastocysts.
METHODS:
The blastocysts of hybrid F1 females [(C57Bl/10 J × CBA-H);N = 15] aged 2-3 months were exposed into the pressure impulse lasting ~0.021 s and characterized by a positive pressure peak of ~76 mmHg. The nuclear DNA fragmentation index of mouse blastocysts was assessed by TUNEL assay within 60 s after exposure to pressure impulse.
RESULTS:
The mean nuclear DNA fragmentation index was significantly higher in the experimental group (83%) than in the control group (19.7%); p < 0.001.
CONCLUSION(S):
A low magnitude pressure impulse can induce nuclear DNA fragmentation in mouse blastocysts. The compression and decompression forces appearing during pressure fluctuations are responsible for the observed DNA shearing.
Pressure induced nucleus DNA fragmentation.
Abstract
PURPOSE:
The present study was designed to investigate the impact of pressure on nuclear DNA integrity in viable cells of mouse blastocysts.
METHODS:
The blastocysts of hybrid F1 females [(C57Bl/10 J × CBA-H);N = 15] aged 2-3 months were exposed into the pressure impulse lasting ~0.021 s and characterized by a positive pressure peak of ~76 mmHg. The nuclear DNA fragmentation index of mouse blastocysts was assessed by TUNEL assay within 60 s after exposure to pressure impulse.
RESULTS:
The mean nuclear DNA fragmentation index was significantly higher in the experimental group (83%) than in the control group (19.7%); p < 0.001.
CONCLUSION(S):
A low magnitude pressure impulse can induce nuclear DNA fragmentation in mouse blastocysts. The compression and decompression forces appearing during pressure fluctuations are responsible for the observed DNA shearing.
沒有留言:
張貼留言