囊胚解凍後須在1小時內(平均0.7小時)重新膨脹，
囊胚解凍後須在3小時內(平均2.1小時)膨脹完全，

J Assist Reprod Genet. 2016 Dec 9. [Epub ahead of print]

Morphokinetics of vitrified and warmed blastocysts predicts implantation potential.

Ebner T1, Oppelt P2, Radler E2, Allerstorfer C2, Habelsberger A2, Mayer RB2, Shebl O2.

Author information

Abstract

PURPOSE:

It was studied whether morphokinetics of blastocoele re-expansion and hatching in vitrified-warmed blastocysts is predictive of implantation, clinical pregnancy, and live birth.

METHODS:

In 144 patients aiming for single warmed blastocyst transfer, blastocysts were cultured in a new time-lapse system (Miri® TL) immediately after warming. Video sequences with an image interval of 5 min were annotated and the corresponding morphokinetic variables were correlated with pregnancy outcome. In detail, tRE (start of re-expansion), tCRE (completion of re-expansion), tAH (hatching from the manipulated zona pellucida), and presence of collapses were recorded.

RESULTS:

In the pregnant group, tRE and tCRE were significantly lower (0.69 ± 0.45 h and 2.16 ± 0.94 h) as compared to the non-pregnant group (1.23 ± 1.08 h and 2.70 ± 1.20 h). Both variables and the duration of re-expansion (tCRE-tRE) allowed for distinction between "non-pregnant," "loss of pregnancy," and live birth/ongoing pregnancy. Presence and number of collapses showed no correlation with outcome.

CONCLUSIONS:

Time-lapse imaging of vitrified-warmed blastocysts offers additional selection criteria allowing for prediction of implantation potential. As a consequence, cumulative pregnancy rate could be increased and time-to-pregnancy reduced.