優質胚胎須在授精
26h內完成第一次分裂,
38h內完成第二次分裂,
80h內完成胚葉融合，
96h內完成囊胚腔形成

J Assist Reprod Genet. 2017 Jun 1. doi: 10.1007/s10815-017-0962-y. [Epub ahead of print]

Selection of human blastocysts with a high implantation potential based on timely compaction.

Mizobe Y1,2, Ezono Y3, Tokunaga M3, Oya N3, Iwakiri R3, Yoshida N3, Sato Y3, Onoue N3, Miyoshi K4.

Author information

Abstract

PURPOSE:

Recently, we established a noninvasive system for selecting human blastocysts with a high pre-transfer implantation potential based on first and second division patterns. The present study was carried out to improve the selection system.

METHODS:

Embryos that completed first and second divisions within 25.90 and 37.88 h after culture, respectively, were selected using a time-lapse incubator. We examined the effects of compaction and blastocyst formation times on pregnancy rates after transferring these embryos at the blastocyst stage.

RESULTS:

The completion of compaction and blastocyst formation times (79.93 and 97.47 h after culture, respectively) of embryos resulting in pregnancies after transfer were significantly (P < 0.01) shorter than those (86.46 and 100.34 h after culture, respectively) of embryos that failed to induce pregnancies. Embryo selection based on completion of compaction time improved pregnancy rates (40.9 vs. 74.6%, P < 0.01).

CONCLUSIONS:

Of the embryos that formed two cells during the first division within 25.90 h after culture and four cells during the second division within 37.88 h after culture, those that completed compaction within 79.93 h after culture before reaching the blastocyst stage had a high implantation potential.