試管嬰兒體外培養過程污染之可能原因為精液造成之感染

試管嬰兒體外培養中，造成培養過程污染之可能原因為精液造成之感染

以 Percoll 離心之方式分離精蟲並無法完全防止感染之發生

Fertil Steril. 1996 Sep;66(3):481-3.

Yeast infection of sperm, oocytes and embryos after intravaginal culture forembryo transfer.

Mahadevan MM, Batres F, Miller MM, Moutos DM.

Source

Department of Obstetrics and Gynecology, University of Arkansas for Medical Sciences.

Abstract

OBJECTIVE:

To report a case of an embryo culture infected with Candida albicans after intravaginal culture.

DESIGN:

Case report.

SETTING:

Private infertility practice and university medical center.

PATIENT:

A couple with tubal and male factor infertility.

INTERVENTIONS:

Superovulation, oocyte recovery, Percoll sperm preparation, and intravaginal culture of sperm and oocytes in a tissue culture tube.

MAIN OUTCOME MEASURES:

Yeast infection of sperm, oocyte, and embryo culture.

RESULTS:

Candida albicans infection occurred in the sperm, oocyte, and embryo culture when cultured in a sealed tube in the vagina. Candida albicans also was found in the prepared sperm suspension culture in a separate tube kept in a 37 degrees C incubator.

CONCLUSIONS:

Infection of the embryo culture with C. albicans probably occurred when contaminated sperm was added at the time of insemination. Sperm preparation by the Percoll gradient centrifugation failed to eliminate C. albicans in the semen.