經過12小時培養
觀察精蟲有無穿透卵子
>10% 正常
<10% 異常
異常不代表精蟲無法穿越人類卵子細胞
The capacity of each sperm to
penetrate the egg membrane and unpack the tightly-coiled DNA found in its head
can be assessed with the SPA or sperm penetration assay. Inactive thawed hamster
eggs are used in this assay. The number of decondensed sperm heads in each egg
is counted as shown in this photo on the left (arrows at 2 of the many heads).
The DNA in each head is visible as a green circle under fluorescence microscopy
shown on the right. Patients with low egg penetration may need sperm injection
during IVF to overcome penetration problems.
http://lomalindahealth.org/health-care/our-services/fertility/conditions-and-treatments/andrology-tour.page
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