卵巢皮質切成極小薄片有利於冷凍保存及再植入

癌症化療病患，可將卵巢皮質部組織切成極小薄片(厚度僅300–350 µM thickness)冷凍保存，
優點為比大切片更有利於冷凍保存
再植後亦比大切片有利於組織與人體產生微血管循環

http://humrep.oxfordjournals.org/content/26/5/1097.full

Micro-organ ovarian transplantation enables pregnancy: a case report

1. Ariel Revel1,*, 
2. Neri Laufer1, 
3. Assaf Ben Meir1, 
4. Meital Lebovich1 and
5. Eduardo Mitrani2

+Author Affiliations

1. ¹Department of Obstetrics and Gynecology, Hadassah Medical Center and Hebrew University-Hadassah Medical School, P.O.Box 12000, Jerusalem 91120, Israel
2. ²Department of Cell and Developmental Biology, Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel

1. *↵Correspondence address. Tel: +97226776389; E-mail: arielr2@hadassah.org.il,arielrevel2@gmail.com

• Received September 22, 2010.
• Revision received February 2, 2011.
• Accepted February 11, 2011.

 

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Abstract

A 19-year-old thalassemic woman had tissue from one of her ovaries cryopreserved prior to bone marrow transplantation, total body irradiation and sterilizing chemotherapy. As expected, premature ovarian failure resulted from this treatment. Transplantation of her thawed ovarian tissue resulted in return of menstrual cycling and the patient then underwent several IVF cycles. The patient, however, had poor ovarian response to hyperstimulation. We thus considered an alternative approach based on the observation that very thin ovarian fragments that preserve the basic ovarian structure [ovarian micro-organs (MOs)] induce angiogenesis and remained viable after autologous transplantation in animals. We report that preparation of autologous tiny ovarian fragments (MO)s and reimplantation into our patient resulted in IVF pregnancy and delivery of a healthy baby.

Figure 2

Alternative method to prepare very thin human ovarian fragments that preserve the ovary's main cortex structure for transplantation. The specially designed apparatus (A and B) cuts 350-µM thick MOs which basically preserve both the length and the depth of the original sliver. Comparison of fresh (C) and frozen–thawed (E) manually cut cortex resembles fresh the comparison of fresh (D) and frozen–thawed (F) cortex cut to MOs.