2013年4月3日

玻璃化冷凍對於卵細胞之代謝產物無明顯影響

本研究顯示，玻璃化冷凍對於卵細胞之代謝產物(tryptophan, phenylalanine, and 2,5,7,8-tetramethyl-2-(2'-carboxyethyl)-6-hydroxychroman (alpha-CEHC) )無明顯影響

http://www.ncbi.nlm.nih.gov/pubmed/23102858

2013 Feb;99(2):565-72. doi: 10.1016/j.fertnstert.2012.09.034. Epub 2012 Oct 23.

Effect of vitrification on human oocytes: a metabolic profiling study.

Dominguez F, Castello D, Remohí J, Simón C, Cobo A.

Source

Fundación IVI-Instituto Universitario IVI, University of Valencia, Valencia, Spain. francisco.dominguez@ivi.es

Abstract

OBJECTIVE:

To evaluate the effect of oocyte vitrification in the metabolomic profile of embryos developed from vitrified and fresh oocytes in our ovum donation program.

DESIGN:

Analysis of the metabolic profiles of spent culture medium samples corresponding to embryos developed from vitrified and fresh oocytes.

SETTING:

In vitro fertilization (IVF) unit/metabolomic facility.

PATIENT(S):

Oocyte donors between the ages of 18 and 35 years.

INTERVENTION(S):

Metabolomic profile liquid chromatography coupled with mass spectrometry (LC-MS) of spent media samples.

MAIN OUTCOME MEASURE(S):

Identification of spent media components and metabolites present and absent in vitrified and fresh day-3 embryos.

RESULT(S):

We obtained a total of 190 spent media samples: vitrification group (65), fresh group (59), and a matched control media group (66). Multivariate data analysis was performed after global metabolomic and amino acid profiles did not reveal any statistically significant differences in day-3 embryos derived from fresh and vitrified oocytes, indicating that other metabolic differences between the samples (e.g., patient-to-patient variability, analytical variation) are greater than those between the vitrified and fresh sample groups. Univariate statistical analysis revealed a series of possible biomarkers, such as tryptophan, phenylalanine, and 2,5,7,8-tetramethyl-2-(2'-carboxyethyl)-6-hydroxychroman (alpha-CEHC), although only alpha-CEHC was statistically significant after correction for multiple testing.

CONCLUSION(S):

Multivariate data analysis did not reveal statistically significant differences between the analyzed groups, suggesting that oocyte vitrification does not disturb embryonic metabolomic profiles.

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