胚胎切片TEcell 5個細胞即足夠
品質較差之囊胚期胚胎(B 或C級)施行胚胎切片TEcell較多(>6個細胞)，雖不影響其囊胚存活率卻可能會影響其著床率

Fertil Steril. 2016 Jan 25. pii: S0015-0282(16)00046-7. doi: 10.1016/j.fertnstert.2016.01.011. [Epub ahead of print]

The number of biopsied trophectoderm cells is likely to affect the implantation potential of blastocysts with poor trophectoderm quality.

Zhang S1, Luo K2, Cheng D1, Tan Y2, Lu C2, He H3, Gu Y2, Lu G4, Gong F2, Lin G5.

Author information

Abstract

OBJECTIVE:

To evaluate whether the developmental potential of the blastocyst is affected by the number of trophectoderm (TE) cells biopsied in preimplantation genetic diagnosis (PGD) cycles.

DESIGN:

Retrospective study.

SETTING:

University-affiliated center.

PATIENT(S):

Women underwent PGD cycles of blastocyst biopsy and fluorescence in situ hybridization analysis.

INTERVENTION(S):

Not applicable.

MAIN OUTCOME MEASURE(S):

Biopsied TE cell number of blastocysts, survival, and implantation rates.

RESULT(S):

The biopsied TE cell number was affected by the TE quality and experience of different embryologists. The diagnostic efficiency increased when from one to five cells were biopsied (86.7%, 91.7%%, 96.0%, 96.8%, to 98.7%) and was maximized when more than six cells were biopsied. To compare the clinical efficiencies, blastocysts were divided into four groups according to biopsied TE cell number: 1-5, 6-10, 11-15, and 16-41. For the blastocysts with grade A TE score, no significant difference was observed in the survival and implantation rates among the four groups. For the blastocysts with grades B and C TE scores, the survival rates showed no significant differences among the four groups, but a significant decreasing trend in implantation rates was observed with increasing biopsied TE cell number.