含20%Fetal calf serum 之冷凍液可達最佳冷凍效果
卵子細胞外圍含部分卵丘細胞可提高胚胎品質

Sci Rep. 2016 Jan 19;6:19465. doi: 10.1038/srep19465.

High survival of mouse oocytes using an optimized vitrification protocol.

Zhou CJ1, Wang DH1, Niu XX1, Kong XW1, Li YJ1, Ren J1, Zhou HX1, Lu A1, Zhao YF1, Liang CG1.

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Abstract

The method of vitrification has been widely used for cryopreservation. However, the effectiveness of this method for mammalian oocytes could be improved by optimizing each step of the process. In the present study, we tested the effects of varying several key parameters to determine the most effective protocol for mouse oocyte vitrification. We found that cryoprotectant containing ethylene glycol and dimethylsulfoxide plus 20% fetal calf serum produced the highest rates of oocyte survival, fertilization, and blastocyst formation. The duration and temperature of oocyte exposure tovitrification and thawing solutions influenced survival rate. The presence of cumulus cells surrounding oocytes and the incubation of thawed oocytes in Toyoda-Yokoyama-Hosoki medium also increased oocyte survival. Open pulled straw and nylon loop methods were more effective than the mini-drop method. Finally, the combination of these improved methods resulted in better spindle morphology when compared to the unimproved methods. These results demonstrate that the outcomes of mouse oocyte vitrification can be improved by a suitable combination of cryopreservation methods, which could be applied to future clinical research with human oocytes.

Figure 3

Effect of concentrations of fetal calf serum (FCS) on OCCs.