VS,TS均25度可達最佳卵子存活率(優於 VS 37度 or TS 37度)

VS: 冷凍液
TS: 解凍液

37度反而增加冷凍液之毒性

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4726034/

Effect of temperature of exposure to VS and TS on OCCs.

Oocytes were exposed to different temperatures of VS (25 °C or 37 °C) and TS 

Spindles morphology was improved by employing the optimized key parameters during vitrification.

(A) Different vitrification procedure used in improved and unimproved groups. (B) Representative images of spindles in improved and unimproved group. Green: TUBB; Blue: DNA; Merge: overlay of TUBB and DNA. Scale bar = 10 μm. (C) Oocytes in improved group get higher percent of normal spindles than that in unimproved group. Different superscript letters (a,b) indicate p < 0.01