slush氮比傳統液態氮更能有效保存卵巢組織

Fertil Steril. 2016 Feb 19. pii: S0015-0282(16)00084-4. doi: 10.1016/j.fertnstert.2016.01.043. [Epub ahead of print]

Successful slush nitrogen vitrification of human ovarian tissue.

Talevi R1, Barbato V2, Fiorentino I2, Braun S2, De Stefano C3, Ferraro R4, Sudhakaran S2, Gualtieri R2.

Author information

Abstract

OBJECTIVE:

To study whether slush nitrogen vitrification improves the preservation of human ovarian tissue.

DESIGN:

Control vs. treatment study.

SETTING:

University research laboratory.

PATIENT(S):

Ovarian biopsies collected from nine women (aged 14-35 years) during laparoscopic surgery for benign gynecologic conditions.

INTERVENTION(S):

None.

MAIN OUTCOME MEASURE(S):

Ovarian cortical strips of 2 × 5 × 1 mm were vitrified with liquid or slush nitrogen. Fresh and vitrified cortical strips were analyzed for cryodamage and viability under light, confocal, and transmission electron microscopy.

RESULT(S):

Compared with liquid nitrogen, vitrification with slush nitrogen preserves [1] follicle quality (grade 1 follicles: fresh control, 50%; liquid nitrogen, 27%; slush nitrogen, 48%); [2] granulosa cell ultrastructure (intact cells: fresh control, 92%; liquid nitrogen, 45%; slush nitrogen, 73%), stromal cell ultrastructure (intact cells: fresh control, 59.8%; liquid nitrogen, 24%; slush nitrogen, 48.7%), and DNA integrity (TUNEL-positive cells: fresh control, 0.5%; liquid nitrogen, 2.3%; slush nitrogen, 0.4%); and [3] oocyte, granulosa, and stromal cell viability (oocyte: fresh control, 90%; liquid nitrogen, 63%; slush nitrogen, 87%; granulosa cells: fresh control, 93%; liquid nitrogen, 53%; slush nitrogen, 81%; stromal cells: fresh control, 63%; liquid nitrogen, 30%; slush nitrogen, 52%).

CONCLUSION(S):

The histology, ultrastructure, and viability of follicles and stromal cells are better preserved after vitrification with slush nitrogen compared with liquid nitrogen.