ICSI過程，卵細胞質過度黏稠之ICSI結果較不佳
胚胎著床率較低
injection funnel: ICSI完後2-3min仍具 funnel
ICSI後胚胎有 injection funnel 預後較差
injection funnel 代表卵細胞質結構有問題
http://humrep.oxfordjournals.org/content/18/6/1294.full
Developmental competence of oocytes showing increased cytoplasmic viscosity
+Author Affiliations
- 1To whom correspondence should be addressed at: Women’s General Hospital, IVF Unit, Lederergasse 47, A‐4010 Linz, Upper Austria, Austria. e‐mail: Thomas.ebner@gespag.at
- Received November 15, 2002.
- Accepted February 7, 2003.
Abstract
BACKGROUND: The objective of the study was to investigate the developmental fate of oocytes with increased cytoplasmic viscosity as assessed by the persistence of the injection funnel after withdrawal of the ICSI pipette. METHODS: For this purpose, 1008 oocytes showing a characteristic injection funnel during ICSI were subdivided into two groups according to the oocyte’s ability to restore its spherical shape within 2–3 min after ICSI. Fertilization and further development was evaluated in both groups. In addition, implantation and pregnancy rates were analysed. RESULTS: In the funnel positive cohort (group 1) significantly fewer oocytes degenerated after injection (P < 0.01) compared with oocytes without persistent funnel (group 2). However, at zygote stage, presence of a halo (P < 0.05) and a optimal pronuclear pattern 0 (P < 0.01) was increased in group 2. In addition, significantly fewer poor quality embryos were found in this group (P < 0.01). The number of good quality blastocysts but not blastocyst formation was increased in group 2 (P < 0.05). This resulted in an increased clinical pregnancy rate if embryos which derived exclusively from funnel negative oocytes were transferred (P < 0.05). CONCLUSIONS: Our data suggest that cytoplasm of higher viscosity delays development up to cleavage stage and impairs optimal development. Injection funnel persistence was found to be a negative prognostic marker of preimplantation development.
Figure 1. (A) MII oocyte showing characteristic injection funnel after withdrawal of glass pipette. (B) Oocyte with immediate restoration of the spherical shape after ICSI. Arrows indicate sperm head and represent 10 µm.
Table I.
Comparison of ICSI outcome between oocytes with persistent funnel and oocytes without
| Persistent funnel | No persistent funnel | P‐value | |
| No. of MII oocytes | 355 | 653 | |
| Fertilization (% 2PN) | 255 (71.8) | 454 (69.5) | NS |
| No fertilization (%) | 77 (21.7) | 116 (17.7) | NS |
| 1PN (%) | 3 (0.9) | 2 (0.3) | NS |
| 3PN (%) | 3 (0.9) | 14 (2.1) | NS |
| Degenerated (%) | 17 (4.8) | 68 (10.4) | <0.01 |
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