卵子體外成熟培養(IVM)重要概念

IVM對象多為PCO

IVM 培養液含75 mIU/mL FSHH & 75 mIU/mL LH


IVM取卵用19g取卵針


pump壓力要調低


micro-TESE 傷口小 切片小，應優於open MESA


IVM卵優先用ICSI


IVM卵子成熟率30%


IVM成熟卵子ICSI懷孕率40-50%


卵泡至少要>6mm才可能IVM


卵泡>9mm， IVM成功率較高



http://www.andrologyjournal.org/cgi/content/full/32/1/23

Live Birth After ICSI of Micro-TESE–Retrieved Spermatozoa Into In Vitro–Matured Oocytes

WEON-YOUNG SON^*, JIN-TAE CHUNG^*, PETER T. K. CHAN AND SEANG LIN TAN^*From the ^* McGill Reproductive Center, Department of Obstetrics and Gynecology, and the  Department of Urology, Royal Victoria Hospital, McGill University Health Centre, Montreal, Quebec, Canada.

Correspondence to: Dr Weon-Young Son, McGill Reproductive Center, Department of Obstetrics and Gynecology, Royal Victoria Hospital, McGill University, Montreal, Quebec H3A 1A1, Canada (e-mail: weon-young.son@muhc.mcgill.ca).

Received for publication March 31, 2010; accepted for publication July 22, 2010.

Abstract
This case report describes a live birth resulting from intracytoplasmic sperm injection (ICSI) of spermatozoa retrieved by microdissection testicular sperm extraction (micro-TESE) into oocytes produced from human chorionic gonadotropin–primed in vitro maturation (IVM) cycles. In the IVM treatment, a total of 30 oocytes (1 mature and 29 immature oocytes) were retrieved. Following IVM, 9 oocytes had matured. A total of 4 oocytes were fertilized after ICSI with the husband's micro-TESE spermatozoa and 4 embryos were transferred into the uterus on day 3. A healthy boy weighing 2500 g was born at 35.5 weeks of gestation.