HA(玻尿酸)對ICSI結果是可能有正面幫助，可提高胚胎品質‧
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2826621/
J Assist Reprod Genet. 2010 Jan;27(1):13-6.
Epub 2009 Dec 30.
Efficiency of hyaluronic acid (HA) sperm selection.
Source
Reproductive Medicine Unit-GynePro Medical Centers, GynePro Medical, Via T. Cremona, 8-40137, Bologna, Italy. l.parmegiani@gynepro.itAbstract
PURPOSE:
Hyaluronic Acid (HA) has a role as "physiologic selector" for spermatozoa prior to intracytoplasmic sperm injection (ICSI). The objective of this study is to analyze the results achievable by the introduction of a routine HA-ICSI programme.METHODS:
We retrospectively observed 293 couples treated with HA-ICSI versus 86 couples treated with conventional PVP-ICSI (historical control group). ICSI was performed on a limited number of oocytes per patient (1-3) according to Italian IVF law at the time of the study. Main outcome measures observed were: fertilization, embryo quality, implantation and pregnancy.RESULTS:
This study showed that Injection of HA-bound spermatozoa (HA-ICSI) significantly improves embryo quality and implantation.CONCLUSIONS:
If wider multi-center randomized studies will confirm these beneficial effects on ICSI outcome, HA could be considered as a routine choice for "physiologic" sperm selection prior to ICSI.Table 1
HA-ICSI versus conventional ICSI
| HA-ICSI | PVP-ICSI | P | |
|---|---|---|---|
| Fertilized oocytes (%) | 874/936 (93.4) | 223/256 (87.1) | 0.533 |
| Grade 1 embryos (%) | 274/778 (35.2) | 48/215 (22.3) | 0.011* |
| Clinical pregnancy rate per transfer (%) | 107/326 (32.8) | 21/96 (21.6) | 0.158 |
| Clinical pregnancy rate per cycle (%) | 107/331 (32.3) | 21/97 (21.6) | 0.163 |
| Implantations (%) | 133/778 (17.1) | 22/213 (10.3) | 0.047* |
| Abortions (%) | 19/107 (17.7) | 3/21 (14.3) | 0.990 |
| No. Ectopic pregnancies | 3 | 0 | |
| No. Gestational sacs without FHB | 3 | 0 | |
| Live births (babies born) | 53 (62) | 18 (19) | |
| Ongoing pregnancies | 29 | 0 |
Difference was considered significant when a P-value was
<0.05
*P-value
<0.05
<0.05For HA-ICSI: on a Petri dish, a 1 µL to 2 µL droplet with suspension of treated spermatozoa (concentration 1
×106) was connected with a
pipette tip to a 5 µL droplet of fresh culture medium (FertiCult Flushing
Medium, FertiPro NV, Beernem, Belgium). Simultaneously, a 5 µL droplet of
HA-containing medium (Sperm Slow™, MediCult) was connected with a pipette tip to
the droplet of fresh culture medium. The spermatozoa on this Petri dish were
incubated for 15 min at 37°C under oil (Liquid Paraffin, MediCult). Spermatozoa
bound to HA in the junction zone of the droplets were selected and easily
detached by injecting pipette (ICSI Micropipets, Humagen Fertility Diagnostics)
and subsequently injected into oocytes. 
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