冷凍卵子對日後受孕形成胚胎細胞代謝無明顯影響

研究顯示冷凍過程對卵子日後受孕形成胚胎細胞之代謝無明顯影響

Fertil Steril. 2012 Oct 23. pii: S0015-0282(12)02253-4. doi: 10.1016/j.fertnstert.2012.09.034. [Epub ahead of print]

Effect of vitrification on human oocytes: a metabolic profiling study.

Dominguez F, Castello D, Remohí J, Simón C, Cobo A.

Source

Fundación IVI-Instituto Universitario IVI, University of Valencia, Valencia, Spain. Electronic address: francisco.dominguez@ivi.es.

Abstract

OBJECTIVE:

To evaluate the effect of oocyte vitrification in the metabolomic profile of embryos developed from vitrified and fresh oocytes in our ovum donation program.

DESIGN:

Analysis of the metabolic profiles of spent culture medium samples corresponding to embryos developed from vitrified and fresh oocytes.

SETTING:

In vitro fertilization (IVF) unit/metabolomic facility.

PATIENT(S):

Oocyte donors between the ages of 18 and 35 years.

INTERVENTION(S):

Metabolomic profile liquid chromatography coupled with mass spectrometry (LC-MS) of spent media samples.

MAIN OUTCOME MEASURE(S):

Identification of spent media components and metabolites present and absent in vitrified and fresh day-3 embryos.

RESULT(S):

We obtained a total of 190 spent media samples: vitrification group (65), fresh group (59), and a matched control media group (66). Multivariate data analysis was performed after global metabolomic and amino acid profiles did not reveal any statistically significant differences in day-3 embryos derived from fresh and vitrified oocytes, indicating that other metabolic differences between the samples (e.g., patient-to-patient variability, analytical variation) are greater than those between the vitrified and fresh sample groups. Univariate statistical analysis revealed a series of possible biomarkers, such as tryptophan, phenylalanine, and 2,5,7,8-tetramethyl-2-(2'-carboxyethyl)-6-hydroxychroman (alpha-CEHC), although only alpha-CEHC was statistically significant after correction for multiple testing.

CONCLUSION(S):

Multivariate data analysis did not reveal statistically significant differences between the analyzed groups, suggesting that oocyte vitrification does not disturb embryonic metabolomic profiles.