未來之趨勢為僅培養少數胚胎, 將多餘胚胎或卵子冷凍

冷凍技術之進步, 未來之趨勢為僅培養少數胚胎, 將多餘之胚胎或卵子冷凍
卵子冷凍後施行ICSI可達類似新鮮卵子之受孕率。

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2854047/

Cumulative ongoing pregnancy rate achieved with oocyte vitrification and cleavage stage transfer without embryo selection in a standard infertility program

Filippo Ubaldi,¹ Reno Anniballo,² Stefania Romano,¹ Elena Baroni,¹ Laura Albricci,¹ Silvia Colamaria,¹ Antonio Capalbo,¹ Fabio Sapienza,¹ Gábor Vajta,³ and Laura Rienzi^1,*

¹G.EN.E.R.A Centre for Reproductive Medicine, Clinica Valle Giulia, Via G. De Notaris 2, 00197 Rome, Italy

²Andrology Center ‘John McLeod’, via F. Petrarca, Naples, Italy

³James Cook University, Cairns Campus, Cairns, QLD, Australia

^*Correspondence address. E-mail: rienzi@generaroma.it

Author information ► Article notes ► Copyright and License information ►

Received December 9, 2009; Revised January 13, 2010; Accepted January 20, 2010.

Copyright © The Author 2010. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Abstract

BACKGROUND

Recent advancement of minimum volume vitrification methods has resulted in a dramatic increase in the efficiency of the process. The aim of this study was to estimate the cumulative reproductive outcome of a cohort of infertile couples undergoing ICSI and oocyte vitrification in restrictive legal conditions, where only a limited number of oocytes could be inseminated per cycle and embryo selection and cryopreservation were forbidden.

METHODS

In this prospective longitudinal cohort study, the cumulative ongoing pregnancy rates obtained by the insemination of fresh and vitrified oocytes from the same cohort were calculated as primary outcome measures. Moreover, the effect of basal and cycle characteristics on clinical outcomes were assessed.

RESULTS

Between September 2008 and May 2009, 182 ICSI cycles were performed where oocyte vitrification was possible. A total of 104 first and 11 second oocyte warming cycles were then performed in non-pregnant patients of the same cohort. The overall ongoing pregnancy rates obtained in the fresh, and first and second warming cycles were 37.4, 25.0 and 27.3%, respectively. The overall cumulative ongoing clinical pregnancy rate observed per stimulation cycle was 53.3%. Maternal age was the only characteristic found to influence the reproductive outcome, with an inverse correlation between the age >40 and the ongoing pregnancy rates (P = 0.04, by Cox regression analysis).

CONCLUSIONS

High cumulative ongoing pregnancy rates can be obtained with transfers of embryos derived from fresh and cryopreserved oocytes in a typical infertile population. Female age significantly affects outcomes in this system.