基因晶片已變成PGD PGS之主流

基因晶片已取代FISH變成PGD/PGS之主流
本篇比較不同microarray之方法
可見之未來，microarray 將變得越來越準確，簡便，快速，
價格可能也會越來越便宜

http://molehr.oxfordjournals.org/content/17/6/335.full

Single-cell whole-genome amplification technique impacts the accuracy of SNP microarray-based genotyping and copy number analyses

1. Nathan R. Treff1,2,*, 
2. Jing Su1, 
3. Xin Tao1, 
4. Lesley E. Northrop1,2 and
5. Richard T. Scott Jr1,2

+Author Affiliations

1. ¹Reproductive Medicine Associates of New Jersey, 111 Madison Ave., Suite 100, Morristown, NJ 07960, USA
2. ²Division of Reproductive Endocrinology, Department of Obstetrics, Gynecology and Reproductive Sciences, UMDNJ-Robert Wood Johnson Medical School, New Brunswick, NJ, USA

1. *↵Correspondence address. Tel: +1-973-871-1254; E-mail: ntreff@rmanj.com

• Received November 24, 2010.
• Revision received December 13, 2010.
• Accepted December 16, 2010.

 

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Abstract

Methods of comprehensive microarray-based aneuploidy screening in single cells are rapidly emerging. Whole-genome amplification (WGA) remains a critical component for these methods to be successful. A number of commercially available WGA kits have been independently utilized in previous single-cell microarray studies. However, direct comparison of their performance on single cells has not been conducted. The present study demonstrates that among previously published methods, a single-cell GenomePlex WGA protocol provides the best combination of speed and accuracy for single nucleotide polymorphism microarray-based copy number (CN) analysis when compared with a REPLI-g- or GenomiPhi-based protocol. Alternatively, for applications that do not have constraints on turnaround time and that are directed at accurate genotyping rather than CN assignments, a REPLI-g-based protocol may provide the best solution.

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