GnRHa或GnRH antagonist對卵顆粒細胞DNA碎片無明顯差異

GnRHa vs GnRH antagonist
對卵子顆粒GC細胞之apoptosis 或DNA碎片並無明顯差異

Eur J Obstet Gynecol Reprod Biol. 2012 Aug 8. [Epub ahead of print]

GnRH agonist versus GnRH antagonist in IVF/ICSI cycles with recombinant LH supplementation DNA fragmentation and apoptosis in granulosa cells.

Lavorato HL, Oliveira JB, Petersen CG, Vagnini L, Mauri AL, Cavagna M, Baruffi RL, Franco JG Jr.

Source

Department of Gynaecology and Obstetrics, Botucatu Medical School, Sao Paulo State University, UNESP, Botucatu, Brazil.

Abstract

OBJECTIVE:

To compare the level of apoptosis and DNA fragmentation in the human granulosa cell (GC) layer exposed to an agonist or antagonist of GnRH in intracytoplasmic sperm injection (ICSI) cycles supplemented with recombinant LH (rLH).

STUDY DESIGN:

Patients without ovulatory dysfunction, aged ≤37years and in their first ICSI cycle were prospectively randomised to receive either a long GnRH agonist protocol or a multi-dose antagonist protocol. In both groups, recombinant FSH supplemented with rLH was used for ovarian stimulation, and the GCs were collected during oocyte denudation. The GCs were then analysed for DNA fragmentation by TUNEL assay and for apoptosis using the annexin-V assay. The outcomes were given as the percentage of GCs with DNA fragmentation and apoptosis out of the total number of GCs analysed. Comparison of the agonist versus the antagonist group was performed using the Mann-Whitney test.

RESULTS:

DNA fragmentation: 32 patients were included in either the GnRH agonist group (n=16) or the antagonist group (n=16). The percentage of GCs with positive DNA fragmentation did not differ significantly (P=0.76) between the agonist group (15.5±9.4%) and the antagonist group (18.8±13.3%). Apoptosis: 28 patients were included in either the GnRH agonist group (n=14) or the antagonist group (n=14). The percentage of GCs positive for apoptosis did not differ significantly (P=0.78) between the agonist group (34.6±14.7%) and the antagonist group (36.5±22%).

CONCLUSIONS:

The results suggest that therapy with either an agonist or antagonist of GnRH is associated with comparable levels of DNA fragmentation and apoptosis in granulosa cells in ICSI cycles supplemented with rLH.