精蟲冷凍儲存時間長短不影響解凍後之精蟲品質

精蟲冷凍儲存時間長短不影響解凍後之精蟲品質
精蟲儲存straw容量亦會影響解凍後之精蟲品質 (0.5ml優於0.2ml)

http://humrep.oxfordjournals.org/content/25/5/1097.full

Long-term cryostorage of sperm in a human sperm bank does not damage progressive motility concentration

1. Leah Yogev1,*, 
2. Sandra E. Kleiman1, 
3. Esther Shabtai2, 
4. Amnon Botchan1,
5. Gedalia Paz1, 
6. Ron Hauser1, 
7. Ofer Lehavi1, 
8. Haim Yavetz1 and 
9. Ronni Gamzu1

+Author Affiliations

1. ¹The Institute for the Study of Fertility, Lis Maternity Hospital, Sackler Faculty of Medicine, Tel Aviv Sourasky Medical Center, 6 Weizman Street, Tel Aviv 64239, Israel
2. ²Statistic Service, Sackler Faculty of Medicine, Tel Aviv Sourasky Medical Center, Tel Aviv University, Tel Aviv, Israel

1. *Correspondence address. E-mail: layogev@zahav.net.il

• Received November 13, 2009.
• Revision received January 19, 2010.
• Accepted January 26, 2010.

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Abstract

BACKGROUND The use of quarantined cryopreserved semen is mandatory in donor insemination programs. Whether sperm cells can survive and retain their ability to fertilize after long-term storage remains a controversial issue. The objective of this study was to determine the effect of the duration of cryostorage in liquid nitrogen on the sperm cells’ progressive motility concentration (PMC) in a large study group.

METHODS A total of 2525 thawed sperm specimens, packed in straws and donated by 72 sperm bank donors for intrauterine insemination (IUI), were evaluated in an assisted reproduction institute. PMC was recorded after 0.5–14.4 years of cryostorage.

RESULTS The mean (±SD) value of PMC of all study samples was 10.8 ± 3.3 × 10⁶/ml after freezing/thawing and before cryostorage (T0), and 12.3 ± 2.9 × 10⁶/ml after storage and before using the specimen for IUI (T1, P < 0.0001). Specimen storage for different lengths of time revealed that storage duration had no significant influence on the PMC of the specimens (r = −0.03, P = 0.08). The PMC of partially filled straws was lower than in full straws. Cryostorage duration made no difference in the PMC of raw and washed sperm specimens.

CONCLUSION Prolonged storage of donated sperm in liquid nitrogen had no influence on the PMC of the specimens and therefore should not alter the fertilization potency of donated sperm. The high post-storage values of the PMC compared with the pre-storage PMC values was probably an artifact of the small volume of the pre-storage sample.