玻璃化冷凍vitrification配合單一胚胎植入可減少PGD之使用率

極體PB基因檢測採用 array comparative genomic hybridization technique (aCGH)

比使用FISH可達到更高之準確性與效率

玻璃化冷凍vitrification配合單一胚胎植入可減少胚胎著床前基因診斷(PGD)之使用率

http://humrep.oxfordjournals.org/content/27/4/951.full

Embryo selection in IVF: is polar body array comparative genomic hybridization accurate enough?

1. Paul N. Scriven1,2,*, 
2. Caroline Mackie Ogilvie2,3 and 
3. Yacoub Khalaf2,4

+Author Affiliations

1. ¹Division of Genetics and Molecular Medicine, King's College London, School of Medicine at Guy's, King's College and St Thomas’ Hospitals, London SE1 9RT, UK
2. ²Guy's and St Thomas’ Centre for PGD, Guy's and St. Thomas’ Hospital NHS Foundation Trust, London, UK
3. ³Cytogenetics Department, Guy's and St Thomas’ NHS Foundation Trust, 5th Floor Tower Wing, Great Maze Pond, London SE1 9RT, UK
4. ⁴Assisted Conception Unit, Guy's and St. Thomas’ Hospital NHS Foundation Trust, London, UK

1. ↵*Correspondence address.  E-mail: paul.scriven@kcl.ac.uk

• Received November 1, 2011.
• Revision received December 28, 2011.
• Accepted January 11, 2012.

 

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Abstract

The emergence of the array comparative genomic hybridization technique (aCGH) is considered an advance in preimplantation genetic testing. Analysis of the recently published pilot study using polar body aCGH indicates that the test accuracy compares favourably with the fluorescencein situ hybridization technique although a substantial number of euploid zygotes are still likely to be excluded incorrectly. A sound argument against selection in principle has recently been published, based on accumulating evidence that potentially all embryos can now be cryopreserved and transferred in subsequent frozen replacement cycles without impairing pregnancy rates. We suggest that vitrification and serial transfer without testing are likely to give patients the best chance for a successful pregnancy, and avoid the use of an expensive technology.