精蟲DNA受損易導致胚胎品質異常

精蟲DNA受損易導致胚胎品質異常，包括胚葉異常多核，早期懷孕流產

http://humrep.oxfordjournals.org/content/20/12/3476.full

Potential adverse effect of sperm DNA damage on embryo quality after ICSI

1. Armand Zini ¹ , ⁴ , 
2. James Meriano ² , 
3. Karim Kader ¹ , 
4. Keith Jarvi ¹ ,
5. Carl A. Laskin ² , ³ and 
6. Kenneth Cadesky ² , ³

+Author Affiliations

1. ¹Division of Urology, Department of Surgery, Mount Sinai Hospital, Toronto, Ontario, ²LifeQuest Centre for Reproductive Medicine and ³Departments of Medicine, Immunology, and Obstetrics and Gynecology, University of Toronto, Toronto, Ontario, Canada

1. 4To whom correspondence should be addressed at: St Mary’s Hospital, 3830 Lacombe Ave., Montreal, Quebec, Canada H3T 1M5. E-mail:armand.zini@ssss.gouv.qc.ca

• Received January 18, 2005.
• Revision received June 6, 2005.
• Accepted June 16, 2005.

 

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Abstract

BACKGROUND: Sperm DNA damage is prevalent amongst infertile men and has been shown to strongly impact adversely natural reproduction, intrauterine insemination-assisted reproduction and to a lesser degree IVF/ICSI fertilization. The objective of this study was to examine further the relationship between sperm DNA denaturation (DD) and reproductive outcomes after ICSI. METHODS: We evaluated infertile couples (n = 60) undergoing IVF/ICSI at a single centre. Sperm DD was assessed by flow cytometry analysis of Acridine Orange-treated sperm and expressed as the percentage of sperm with DD. Couples were sub-grouped according to sperm DD results: group 1: 0–15%; group 2: >15–30%; group 3: >30%. RESULTS: There were no differences between the three groups with regard to maternal age, sperm parameters, oocyte maturation, fertilization or pregnancy rates. Group 3 had a significantly higher rate of multinucleation among the embryo cohorts compared to either groups 1 or 2 (20% versus 10% and 8% respectively, P = 0.04). There was a statistically insignificant trend toward an increased spontaneous pregnancy loss rate in group 3 (P =0.50). CONCLUSION: Although we did not observe significant relationships between sperm DNA damage and either fertilization or pregnancy rates, the potential adverse effect of sperm DNA damage on embryo quality and spontaneous pregnancy loss is concerning.