無中空透明帶(ZP)卵子仍可ICSI受孕, 培養至囊胚, 冷凍

Fertil Steril. 2015 May;103(5):e35. doi: 10.1016/j.fertnstert.2015.02.016. Epub 2015 Mar 13.

Time-lapse monitoring of zona pellucida-free embryos obtained through in vitro fertilization: a retrospective case series.

Bodri D1, Kato R2, Kondo M2, Hosomi N2, Katsumata Y2, Kawachiya S2, Matsumoto T2.

Author information

Abstract

OBJECTIVE:

To report time-lapse monitoring of human oocytes in which the damaged zona pellucida was removed, producing zona-free (ZF) oocytes that were cultured until the blastocyst stage in time-lapse incubators.

DESIGN:

Retrospective case series.

SETTING:

Private infertility clinic.

PATIENT(S):

Infertile patients (n = 32) undergoing minimal ovarian stimulation or natural cycle IVF treatment between October 2012 and June 2014.

INTERVENTION(S):

Intracytoplasmic sperm injection (ICSI) fertilization of ZF oocytes, prolonged embryo culture in time-lapse incubators, elective vitrification, and subsequent single vitrified-thawed blastocyst transfer (SVBT).

MAIN OUTCOME MEASURE(S):

Rate of fertilization, cleavage and blastocyst development, live-birth rate per SVBT cycle.

RESULT(S):

In spite of advanced maternal age (39 ± 4.2; range, 30-46 years), good fertilization (94%), cleavage (94%), and blastocyst development rates (38%) were reached after fertilization and culturing of ZF oocytes/embryos. All thawed ZF blastocysts survived, and up to this date seven SVBT transfers were performed, yielding three (43%) term live births with healthy newborns.

CONCLUSION(S):

Time-lapse imagery gives a unique insight into the dynamics of embryo development in ZF embryos. Moreover, our case series demonstrate that an oocyte with a damaged zona pellucida that has been removed could be successfully fertilized with ICSI, cultured until blastocyst stage in a time-lapse incubator and vitrified electively for subsequent use.