精蟲保存方法中,  慢速冷凍優於玻璃化冷凍法

Cryo Letters. 2016 Mar-Apr;37(2):123-8.

Comparative effects of slow freezing and vitrification on cryosurvival of spermatozoa obtained from west African dwarf goat bucks.

Daramola JO1, Adekunle EO2.

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Abstract

BACKGROUND:

Slow freezing and vitrification are used to improve the viability of spermatozoa from various species but comparative effects of these cryoprotocols have never been evaluated for spermatozoa obtained from West African Dwarf (WAD) goat bucks.

OBJECTIVE:

This study evaluated the comparative effects of slow freezing and vitrification on the viability of spermatozoa of WAD goat bucks.

MATERIALS AND METHODS:

Semen samples collected with the aid of artificial vagina were allocated to slow freezing and vitrification protocols and cryopreserved for 30 days in liquid nitrogen.

RESULTS:

Consistent higher (P<0.05) sperm motility, acrosome integrity, membrane integrity, live sperm, acrosome reaction and capacitation in slow freezing compared to vitrification. Abnormal sperm cells and malondialdehyde (MDA) concentrations reduced (P<0.05) in slow freezing compared to vitrification. Higher (P<0.05) arginase activity was observed in slow freezing compared to vitrification.

CONCLUSION:

The findings indicated that spermatozoa obtained from WAD goat bucks were better preserved in slow freezing compared tovitrification.