無精症睪丸切片TESE後完全不動精蟲可經由4方法挑選具生命力精蟲
(1)半滲透壓溶劑---活精蟲尾部呈現捲曲
(2)化學藥劑刺激---活精蟲尾部呈現振動
(3)精蟲尾部彎曲性---活精蟲頭部尾部長軸呈現不同方向
(1)精蟲尾部雷射反應---活精蟲尾部單發雷射後呈現捲曲

Andrology. 2015 Mar;3(2):156-62. doi: 10.1111/andr.286. Epub 2014 Oct 20.

How to select immotile but viable spermatozoa on the day of intracytoplasmic sperm injection? An embryologist's view.

Nordhoff V1.

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Abstract

A viable spermatozoon is the prerequisite for initiating fertilization in intracytoplasmic sperm injection. Usually motility is the primary sign used to determine a sperm's viability. However, in every in vitro fertilization (IVF) laboratory cases are observed in which none or only few spermatozoa are motile. This can occur in treatment cycles involving ejaculated samples but is most common in cases where surgically extracted testicular spermatozoa are used. To aid in selection, several techniques have been developed to identify viable spermatozoa from the immotile fraction. Amongst the more commonly used approaches are (i) the hypo-osmotic swelling test (ii) chemical substances for induction of tail movements (iii) the sperm tail flexibility test and (iv) laser-assisted immotile sperm selection. All can be used routinely in an IVF laboratory with each having both strengths and weaknesses. It is the purpose of this short review to focus on the technical issues involved in the performance of each of these techniques and to highlight the advantages and disadvantages of each approach.

© 2014 American Society of Andrology and European Academy of Andrology.